Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (7): 1212-1219.doi: 10.3969/j.issn.2095-4344.2013.07.014
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Wang Min, Cao Bing-zhen
Received:
2012-05-11
Revised:
2012-06-18
Online:
2013-02-12
Published:
2013-02-12
Contact:
Cao Bing-zhen, Doctor, Chief physician, Department of Neurology, Jinan Military General Hospital, Jinan 250031, Shandong Province, China
cbzxia2011@163.com
About author:
Wang Min☆, Studying for doctorate, Attending physician, Department of Neurology, Jinan Military General Hospital, Jinan 250031, Shandong Province, China
xmeht@163.com
CLC Number:
Wang Min, Cao Bing-zhen. Glial cell responses and matrix metalloproteinase-9 expression in the brain of chronic vascular dementia rat models[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1212-1219.
2.3 各组大鼠脑组织学观察 苏木精-伊红、KB及Bodian染色:见图2-4。模型组大鼠缺血1-3 d,额颞叶皮质及海马区锥体细胞缺血水肿呈三角形,缺血7-14 d,皮质下及脑室周围出现较多梗死灶,以海马及新皮质的3,5,6层神经细胞及尾状核等纹状体区神经细胞坏死最明显。可见较多炎性细胞浸润,主要是单核吞噬细胞,海马CA1区锥体细胞及齿状回细胞减少。缺血一两个月除上述表现外,脑室周围白质疏松呈空泡样变,海马区锥体细胞严重脱失、基质疏松及微空泡形成,星形胶质细胞明显增生,白质疏松更加明显。KB染色可见髓鞘脱失,Bodian染色示轴索连续性完好。缺血三四个月,白质疏松更加明显,髓鞘脱失明显,Bodian染色未见轴索断裂,纤维性星形胶质细胞明显增多。可见部分炎性细胞围绕小静脉周围形成袖套样改变。 免疫组织化学染色:见图5-7。正常组及假手术组大鼠未发现阳性小胶质细胞,未见基质金属蛋白酶9表达,在皮质及白质内可见散在星形胶质细胞。缺血1 d,皮质、基底节、内囊、胼胝体、海马、视束等处均有胞浆染色阳性的小胶质细胞;基质金属蛋白酶9表达在缺血3-7 d以海马CA1区增加明显;缺血7-14 d脑室周围白质小胶质细胞显著增多,梗死灶周围小胶质细胞增生明显。缺血1个月,小胶质细胞反应达到高峰,小胶质细胞和基质金属蛋白酶9的表达以室周白质最为显著,其次是皮质和海马;梗死中心区阿米巴状、圆状小胶质细胞高度聚集,梗死区周边见高分枝状小胶质细胞;缺血2-4个月后仍以室周白质及白质疏松处最显著,而海马区小胶质细胞活动则减少。胶质纤维酸性蛋白阳性星形胶质细胞在时间、空间分布及反应程度上与小胶质细胞反应相平行,形态上也经历了早期的纤维性星形胶质细胞,到胞体增大、肿胀,树突增粗,胞浆深染的反应性星形胶质细胞,后期则出现较多的纤维性星形胶质细胞。"
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