Abnormal expression of srGAP2 in the brain of rats with refractory epilepsy

doi:10.3969/j.issn.2095-4344.2013.07.013

Abstract

Abstract:

BACKGROUND: Many studies show srGAP2 molecules play a key role in neurite outgrowth and synaptic plasticity, but the exact mechanism is unknown.
OBJECTIVE: To study the possible correlation between srGAP2 expression and epilepsy pathogenesis, through testing the expression of srGAP2 in epileptic rats.
METHODS: Fifty-six Sprague-Dawley male rats were divided randomly into seven groups, including six groups with epilepsy induced by lithium-pilocarpine management and one control group. The samples of temporal lobe tissue were taken from rats at 6 hours, 1 day, 1 week, 2 weeks, 1 month, and 2 months after seizures, and from controls group. Using immunohistochemistry, immunefluorescence, and western-blot methods, we tested the expression of srGAP2 in experimental animals and control groups.
RESULTS AND CONCLUSION: Western blotting of rat brain tissue showed that srGAP2 was up-regulated in the cortex and hippocampus of the epileptic rats as compared with the control rats. The maximal expression was seen at about 2 weeks, and relatively high expression maintained until 1 month. The expression then returned down, and approached normal levels at 2 months. These results were consistent with the immunohistochemical and immunofluorescence results. These data implicate srGAP2 may promote mossy fiber sprouting, and participate in the abnormal development of synapses. The result suggests that this protein may play an important role in the pathogenesis of intractable epilepsy.

Key words: tissue construction, experimental modeling in tissue construction, refractory epilepsy, srGAP2, mossy fiber sprouting, epilepsy models, brain tissue, cellular biology, molecular biology, tissue construction photographs-containing paper

CLC Number:

R318

Liu Jin, Xu Zhong, Pang Ai-lan, Meng Bu-liang, Wang Xue-feng, Ren Hui. Abnormal expression of srGAP2 in the brain of rats with refractory epilepsy[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1207-1211.

Figures/Tables 5

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