Repair of bone defects due to chronic osteomyelitis in rabbits using nano-hydroxyapatite/chitosan scaffold carrying vancomycin/polyactic-co-glycolic acid sustained-release microspheres combined with autologous red bone marrow

doi:10.3969/j.issn.2095-4344.1568

Abstract

Abstract:

BACKGROUND: Preliminary studies have confirmed that vancomycin sustained-release microspheres exert satisfactory therapeutic effect on chronic osteomyelitis in rabbits. Nano-hydroxyapatite/chitosan (nHA/CS) has good biocompatibility, degradability and osteogenic induction. Autologous red bone marrow can be used as a seed cell source combined with nHA/CS scaffold to repair rabbit tibia bone defects. Therefore, the combined effects of the three materials in the repair of rabbit chronic osteomyelitis bone defects need a further investigation.

OBJECTIVE: To explore the efficacy and feasibility of nHA/CS scaffold carrying vancomycin/polyactic-co-glycolic acid (PLGA) sustained-release microspheres combined with autologous red bone marrow in the repair of chronic osteomyelitis bone defects in rabbits.

METHODS: Vancomycin/PLGA sustained-release microspheres were prepared by water oil and water emulsion method. The nHA/CS scaffolds carrying vancomycin/PLGA sustained-release microspheres were prepared by vacuum freeze drying, and mechanical properties and vancomycin release of the scaffold were determined. Twenty 5-month-old New Zealand white rabbits provided by Laboratory Animal Centre of Daping Hospital of Third Military Medical University were selected for establishing the chronic osteomyelitis bone defect model by intramedullary infection of Staphylococcus aureus into the left tibia. The model rabbits were randomly divided into four groups: group A was only given only for debridement treatment; in group B, the nHA/CS scaffold was implanted after debridement; in group C, nHA/CS scaffolds carrying vancomycin/PLGA sustained-release microspheres were implanted after debridement; in group D, nHA/CS scaffolds carrying vancomycin/PLGA microspheres and autologous red bone marrow were implanted after debridement. At 3 months after implantation, the repair effect was observed by X-ray and hematoxylin-eosin staining.

RESULTS AND CONCLUSION: The nHA/CS scaffolds carrying vancomycin/PLGA sustained-release microspheres held good biomechanical properties and drug sustained release. At 3 months after implantation, X-ray revealed that the defect region in the groups A and B had no obvious improvement, and there were still bone destruction and periosteal reaction. The group C showed the formation of the new bone, the defect area was slightly blurred, but the defect still existed. In the group D, new bone formed, the defect area became blurred, the density was slightly high, and the periosteum reaction disappeared. Hematoxylin-eosin staining showed the bone trabecular disorder and more damage in the groups A and B. The bone trabecula was neatly arranged and less damaged in the groups C and D, and especially the group D had thickened bone trabecula arranged regularly. These results indicate that nHA/CS scaffolds carrying vancomycin/PLGA sustained-release microspheres combined with autologous red bone marrow exhibit good outcomes in repairing the bone defect due to chronic osteomyelitis in rabbits.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydroxyapatites, Chitosan, Vancomycin, Microspheres, Osteomyelitis;, Bone Marrow, Tissue Engineering

CLC Number:

Xiang Bingyan, Li Peng, Bo Fan, Zhou Sirui . Repair of bone defects due to chronic osteomyelitis in rabbits using nano-hydroxyapatite/chitosan scaffold carrying vancomycin/polyactic-co-glycolic acid sustained-release microspheres combined with autologous red bone marrow[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(6): 843-848.

Figures/Tables 6

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