Effect of Danshen injection with neural stem cells transplantation in rats with craniocerebral injury

doi:10.3969/j.issn.2095-4344.2017.29.020

Abstract

Abstract:

BACKGROUND: Danshen injection can dramatically enhance the proliferation and survival of neural stem cells (NSCs) after transplantation.
OBJECTIVE: To investigate the effect of Danshen injection with NSCs transplantation in rats with craniocerebral injury.
METHODS: Eighty-seven adult Sprague-Dawley rats were used to build severe traumatic brain injury models. After successfully modeling, the model rats were randomized into craniocerebral injury group (the subarachnoid injection of DMEM medium; n=20), Danshen injection group (the intraperitoneal injection of Danshen injection; n=20), and NSCs group (the subarachnoid injection of CM-Dil-labeled NSCs suspension; n=20) and combined group (intraperitoneal injection of Danshen injection plus the subarachnoid injection of CM-Dil-labeled NSCs suspension; n=20). The injection in each group was performed once a day for 3 consecutive days. The modified neurological severity scores were tested after 3 days, 1, 2, 3 and 4 weeks of the transplantation. The Morris water maze test was performed at 21-28 days of transplantation. NSCs survival and distribution were detected by fluorescence microscope and the pathologic changes of brain tissues were observed at 4 weeks after intervention. The expressions of synaptophysin and growth associated protein 43 at gene and protein levels were tested in the brain injury zone by RT-PCR and western blot assay respectively.
RESULTS AND CONCLUSION: The modified neurological severity scores in the combined group were significantly lower than those in the other groups (P < 0.05). In the Morris water maze test, the average escape latencies in the combined group was significantly shortened in the combined group after 3-5 days of transplantation in comparison with the other three groups (P < 0.05 or P < 0.01); the frequency of passing through the platform and the percentage of the swimming distance at the target quadrant in the total swimming distance were significantly higher in the combined group than in the other groups (P < 0.05 or P < 0.01). The hematoxylin-eosin staining results showed the tissue voids in the Danshen injection and NSCs group were relatively smaller than those in the craniocerebral injury group, and the voids almost disappeared in the combined group. There were more CM-Dil positive cells in the combined group than the NSCs group. RT-PCR and western blot assay showed that the gene and protein expression levels of synaptophysin and growth associated protein 43 in the combined group were the highest followed by NSCs and Danshen injection groups, and the lowest expression levels were observed in the craniocerebral injury group. In summary, the Danshen injection combined with the NSCs transplantation can improve the neurological function of rats with severe craniocerebral injury, which might function by increasing the expression levels of synaptophysin and growth associated protein 43.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Neural Stem Cells, Drugs, Chinese Herbal, Cell Transplantation

CLC Number:

R394.2

Liu Xiao, Wang Hu. Effect of Danshen injection with neural stem cells transplantation in rats with craniocerebral injury[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(29): 4709-4715.

Figures/Tables 2

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