Fibrin glue combined with vascular endothelial growth factor and recombinant human bone morphogenetic protein 2 applied in spinal fusion

doi:10.3969/j.issn.2095-4344.2016.25.008

Abstract

Abstract:

BACKGROUND: Bone morphogenetic protein 2 possesses osteoinduction, and vascular endothelial growth factor plays a positive regulatory role in osteogenesis and bone repair. So what will happen if both of them are used for spinal fusion?

OBJECTIVE: To investigate the effects of fibrin glue combined with vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 on intertransverse process fusion.

METHODS: Thirty Japanese white rabbits were randomized into three groups (n=10 per group): rabbits underwent intertransverse process fusion at the level of L_5-6 with vascular endothelial growth factor 165/recombinant human bone morphogenetic protein 2/fibrin glue in experimental group, recombinant human bone morphogenetic protein 2/fibrin glue in control group, and fibrin glue in blank control group, respectively. At 6 weeks after implantation, observations of biomechanics, X-ray imaging and histology were performed.

RESULTS AND CONCLUSION: Osteogenic fusion between transverse processes could be found in experimental and control groups except for the blank control group, which was better in the experimental group. The strength of osteogenic fusion in the experimental group was significantly stronger than that in the control group (P < 0.01). Besides, abundant chondrocytes, osteocytes, mature bone tissues and collagens could be found in the osteogenic fusion specimen of the experimental group, and numerous chondrocytes, osteocytes and mature bone tissues observed in the osteogenic fusion specimen of the control group. In conclusion, fibrin glue combined with vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 can promote osteogenic fusion.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Spinal Fusion, Vascular Endothelial Growth Factors, Bone Morphogenetic Proteins, Tissue Engineering

CLC Number:

R318

Tian Ye, Zhu Yue, Zhong Tao. Fibrin glue combined with vascular endothelial growth factor and recombinant human bone morphogenetic protein 2 applied in spinal fusion[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3699-3705.

Figures/Tables 5

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