Biocompatibility of hydroxyapatite/TiO2 nanotube composites

doi:10.3969/j.issn.2095-4344.2014.03.002

Abstract

Abstract:

BACKGROUND: Hydroxyapatite has excellent biocompatibility, but biocompatibility of nano-hydroxyapatite/TiO₂ nanotube composites is rarely reported.
OBJECTIVE: To evaluate the biocompatibility of nano-hydroxyapatite/TiO₂ nanotube composites.
METHODS: First, the TiO₂nanotubes were fabricated on the surface of the titanium by anodic oxidation technique. Second, the nano-hydroxyapatite/TiO₂nanotube composites were fabricated by electrodeposition technique. The surface morphology of the composites was observed by scanning electron microscopy. Mouse osteoblasts MC-3T3-E1 were co-cultured with the nano-hydroxyapatite/TiO₂ nanotube composites, TiO₂nanotubes and titanium, and commercially pure titanium to observe the cell adhesion, proliferation and necrosis on scaffolds.
RESULTS AND CONCLUSION: The morphology of the TiO₂ nanotubes and nano-hydroxyapatite/TiO₂ nanotube composites could be controlled by altering the conditions of the anodic oxidation and electrodeposition. Under the inverted microscope, after 3 days of co-culture with TiO₂ nanotubes and nano-hydroxyapatite/TiO₂ nanotube composites, MC-3T3-E1 cells proliferated well with regular shape and arrangement that were superior to those on commercially pure titanium. Under scanning electron microscope, the cell were adhered and proliferated well on the surface of the TiO₂nanotubes and nano-hydroxyapatite/TiO₂nanotube composites after 3 days. Apoptosis rate of the cells was significantly reduced on the surface of nano-hydroxyapatite/TiO₂ nanotube composites (7.8%) compared with TiO₂ nanotubes (9.4%) and commercially pure titanium (13.5%), indicating nano-hydroxyapatite/TiO₂ nanotube composites have good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, nanoparticles, nanotubes, durapatit, materials testing

CLC Number:

R318

Zhang Hang-zhou, Sun Yu, Wang Lin, Tian Ang, Xue Xiang-xin, Bai Xi-zhuang. Biocompatibility of hydroxyapatite/TiO₂ nanotube composites[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(3): 335-340.

Figures/Tables 6

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Biocompatibility of hydroxyapatite/TiO₂ nanotube composites

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