Three-dimensional printed polylactic acid resin humerus
combined with bioactive coating promotes osteoblast adhesion and increases antibacterial
ability

doi:10.3969/j.issn.2095-4344.2255

Abstract

Abstract:

BACKGROUND: Polylactic acid has good bio-toughness and biocompatibility, but bio-inertity limits the interaction of polylactic acid resin implants with surrounding tissues after implantation in vivo. Therefore, research on improving the biological activity of polylactic acid resin and improving bone binding properties has gradually become a hotspot of biological materials.

OBJECTIVE: To observe the effects of polylactic acid resin combined with different bioactive coatings on the adhesion, proliferation and differentiation of osteoblasts, and to analyze their antibacterial ability.

METHODS: The three-dimensional printed polylactic acid biomimetic tibia specimens were made, and chitosan-α-β-GP thermosensitive hydrogel coating and chitosan nanoparticle coating were prepared on the surface. MC3T3-E1 cells were inoculated separately into three-dimensional printed polylactic acid biomimetic tibia (blank group), chitosan-α-β-GP thermosensitive hydrogel coated three-dimensional printed polylactic acid biomimetic tibia (hydrogel group) and shell-containing coating three-dimensional printed polylactic acid biomimetic tibia (nanoparticle group) surface. The cell adhesion, proliferation, and differentiation were observed. The cell adhesion plaque and osteoprotegerin gene and protein expression levels, and the expression levels of p65 and p-p65 were detected. The interleukin-6 secretion was detected. Gram-positive and Gram-negative bacteria were inoculated on the surface of the three samples, respectively, and the bacterial adhesion was observed.

RESULTS AND CONCLUSION: (1) After 8 hours of inoculation, the number of adherent cells in the nanoparticle group was higher than that in the hydrogel and blank groups (P < 0.05), and the number of adherent cells hydrogel group was higher than that in the blank group (P < 0.05). (2) Cell viability in the nanoparticle and gel groups was higher than that in the blank group at 2, 4, 6 and 8 days (P < 0.05). (3) After 6 days of culture, the cell adhesion spots and the expression levels of osteoprotegerin protein and mPNA in the particle and hydrogel groups were higher than those in the blank group (P < 0.05), and they were higher in the nanoparticle group than those in the hydrogel group (P < 0.05). (4) After 8 days of culture, the alkaline phosphatase activity and the secretion content of interleukin-6 in the nanoparticle and hydrogel groups were higher than those in the blank group (P < 0.05), and they were higher in the nanoparticle group than those in the hydrogel group (P < 0.05). The level of p-p65 in the nanoparticle group was higher than that in the hydrogel group (P < 0.05), and it was higher in the hydrogel group than that in the blank group (P < 0.05). (5) The antibacterial abilities to Gram-positive and Gram-negative bacteria were as follows: nanoparticle group > hydrogel group > blank group (P < 0.05). (6) In summary, three-dimensional printed polylactic acid humerus combined with α-β-GP thermosensitive hydrogel and chitosan nanoparticles can effectively promote cell adhesion and proliferation and increase alkaline phosphatase activity, the secretion of antibacterial factors, and improve antibacterial ability, which is related to activation of NF-κB signaling pathway.

Key words: three-dimensional printing, polylactic acid, bionic humerus, chitosan, α-β-GP temperature-sensitive hydrogel, chitosan nanoparticles, cell proliferation, anti-bacterial ability

CLC Number:

Zhang Zhongyan, Li Yubo, Qi Tongning, Chang Tao. Three-dimensional printed polylactic acid resin humerus combined with bioactive coating promotes osteoblast adhesion and increases antibacterial ability [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(16): 2485-2492.

Figures/Tables 8

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