Bioprinting of 3D structure with micro-network fluidic channels

doi:10.3969/j.issn.2095-4344.0688

Abstract

Abstract:

BACKGROUND: Constructing a three-dimensional pre-vascularization system plays a decisive role in the survival and functional expression of cells in large and complex three-dimensional structures. Therefore, seeking a suitable pre-vascularization strategy has become a problem to be solved urgently in the 3D bio-printing of large size and complex three-dimensional tissue.

OBJECTIVE: To make a preliminary exploration on the 3D bioprinting of large-scale and pre-vascularized three-dimensional structure.

METHODS: Catia as a reverse engineering software was used to design a three-dimensional biological print blueprint. A modified desktop-grade dual-head 3D printer was used as a biological printer and polyvinyl alcohol as a sacrificial material to print a sacrificial skeleton. The mixture of rat bone marrow mesenchymal stem cells, sodium alginate, agarose and nano-cellulose solution was used as cell biological ink. According to the pre-designed parameters for printing, the tissue engineering three-dimensional structure with three-dimensional fluid channel was constructed. The three-dimensional structure obtained by printing was observed to evaluate the cell activity after printing as well as after material dissolution. The printed structure was cultured in vitro and the proliferation of cells in the three-dimensional structure was detected by Alamar Blue kit.

RESULTS AND CONCLUSION: A double-nozzle printing model with micro-porous outer wall and interlaced microtubules was designed by using Catia software. The three-dimensional biometric printing technology was used to construct the three-dimensional structure with self-defined size (especially its height) and pre-vascularization. The survival rate of the cells in the printed structure within 12 hours was (95.47±0.54)%, and as the in vitro culture time prolonged, the cell survival rate decreased but still exceeded 80%. Over time, the cell proliferation showed an increasing tendency. These findings indicate that this biometric printing method can be used to produce three-dimensional structures with different characteristics by using various bio-hydrogel materials, and has potential in the biofabrication of complex tissues and organs with clinically-related dimensions of pre-vascularization.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Materials Testing, Hydrogel, Cell Proliferation, Tissue Engineering

CLC Number:

R459.9

R318

Zou Qiang, Sun Yu, Li Xuanze, Wu Zhanyu, Yang Long, Wang Jianji, Liu Qin, Ma Minxian, Ye Chuan. Bioprinting of 3D structure with micro-network fluidic channels[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 265-271.

Figures/Tables 5

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