Low-dose reduced glutathione promotes umbilical cord blood mesenchymal stem cell proliferation

doi:10.3969/j.issn.2095-4344.2014.01.011

Abstract

Abstract:

BACKGROUND: How to obtain a sufficient number of cells is one of the key issues in the cell transplantation therapy, and studies have shown that stem cell proliferation can be promoted by reasonable stimulus.
OBJECTIVE: To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells.
METHODS: The cells were divided into two groups: the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione.
RESULTS AND CONCLUSION: At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P < 0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P < 0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.

Key words: stem cells, umbilical cord, mesenchymal stem cells, glutathione

CLC Number:

R394.2

Qu Xin, Di Xu, Han Lu, Zhang Hai-chao. Low-dose reduced glutathione promotes umbilical cord blood mesenchymal stem cell proliferation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 63-68.

Figures/Tables 3

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