Action mechanism of Wuwei Xiaodu Yin in the treatment of periprosthetic joint infection based on network pharmacology

doi:10.3969/j.issn.2095-4344.2906

Abstract

Abstract:

BACKGROUND: Wuwei Xiaodu Yin has been used to treat periprosthetic joint infection in clinic, but its molecular mechanism is not clear due to the complexity of its active ingredients.

OBJECTIVE: To investigate the action mechanism of Wuwei Xiaodu Yin in the treatment of periprosthetic joint infection.

METHODS: The active compounds and targets of Wuwei Xiaodu Yin were obtained by using Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) database. The disease targets corresponding to periprosthetic joint infection were obtained by using GeneCards and OMIM database. The intersection of the two was taken to obtain the Wuwei Xiaodu Yin-periprosthetic joint infection disease intersection target. The STRING online database was used to construct the protein-protein interaction network. Core genes were screened according to degree value. The intersection genes were fed into Cytoscape 3.7.2 software to construct the Wuwei Xiaodu Yin-target-periprosthetic joint infection visual network map. Furthermore, by means of DAVID online tool, the gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of the intersection target were analyzed to explore the actin mechanism of Wuwei Xiaodu Yin control over periprosthetic joint infection.

RESULTS AND CONCLUSION: A total of 37 active compounds were predicted from the BATMAN-TCM platform, with 914 target sites. A total of 286 periprosthetic joint infection target genes were screened from GeneCards and OMIM databases. Totally 39 drug and disease intersection targets were obtained by intersection, and 16 core genes were obtained by screening. (2) GO analysis results showed that the biological function of intersection target mainly included positive regulation of chemokine biosynthetic process, protein kinase B signaling, inflammatory response, lipopolysaccharide-mediated signaling pathway, negative regulation of cytokine secretion involved in immune response and so on. Molecular functions included cytokine activity, growth factor activity, ICAM-3 receptor activity, type III transforming growth factor beta receptor binding, and prostaglandin-endoperoxide synthase activity. Cell composition mainly included extracellular space, cell surface, external side of plasma membrane, extracellular region, and integrin alphaL-beta2 complex. (3) KEGG pathway enrichment showed that above targets were mainly related to rheumatoid arthritis, malaria, cytokine-cytokine receptor interaction, American trypanosomiasis, leishmaniasis, inflammatory bowel disease, amoebiasis, tumor necrosis factor signaling pathway, tuberculosis, and natural killer cell mediated cytotoxicity. (4) The action mechanism of Wuwei Xiaodu Yin in treating periprosthetic joint infection disease is characterized by multiple effective compounds, multiple action pathways and multiple target genes, providing new ideas and new directions for subsequent experiments.

Key words: joint, prosthesis, periprosthetic infection, traditional Chinese medicine, wuweixiaoyin, network pharmacology, pathway, factor

CLC Number:

Zhang Haitao, Xin Pengfei, Feng Wenjun, Cao Houran, Chen Jinlun, Deng Peng, Jie Ke, Ge Yingjie, Peng Xinyu, Li Jie, Zeng Jianchun, Zeng Yirong. Action mechanism of Wuwei Xiaodu Yin in the treatment of periprosthetic joint infection based on network pharmacology[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(36): 5843-5849.

Figures/Tables 9

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