Canine dental pulp stem cells-polyglycolic acid scaffold complex for canine periodontal tissue defect

doi:10.3969/j.issn.2095-4344.2350

Abstract

Abstract:

BACKGROUND: Our previous studies have constructed polyglycolic acid-human dental pulp stem cells complex, and it is shown that mechanical stimulation of the complex can be effective in the construction of tissue-engineered tendons or ligaments.

OBJECTIVE: We assume that tissue-engineered tendons or ligaments constructed by polyglycolic acid and canine dental pulp stem cells may promote the healing of periodontal tissue defect, so we verify the promoting effect of the complex for repairing canine one-wall periodontal defects.

METHODS: One-wall acute periodontal defect models of six Beagle dogs were prepared. The models were randomly assigned to experimental group (canine dental pulp stem cells-polyglycolic acid complex) and control group (without any implanting). Periodontal regeneration was observed by hematoxylin-eosin staining and AZAN staining at 12 weeks after implantation. The animal experiment was approved by the experimental animal welfare and ethics committee of Fuzhou General Hospital of Nanjing Military Area Command.

RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining: There were numerous new bones at the site of the periodontal defect; there were still differences between the new bone structure in the distal part of the root surface defect and the normal bone structure; the fibrous connective tissue above was thick in the experimental group. There was only a small amount of new bone at the site of the defect, and the gingiva and the root surface did not combine to form long junctional epithelium structure in the control group. (2) AZAN staining: Compact new bone formation, periodontal membrane regeneration and thin cementum formation could be seen at the site of the periodontal defect; new periodontal membrane was close to the normal periodontal membrane tissue in the experimental group. There was only a small amount of new bone formation at the site of the defect, almost no new periodontal membrane, and the upper part was gingival connective tissue, and the gingiva and the root surface did not combine to form long junctional epithelium structure in the control group. (3) Histological quantitative analysis results showed that the length of junctional epithelium, new cementum, new periodontal membrane, new bone height, and new bone area were more in the experimental group than in the control group (P < 0.05). (4) These results confirm that canine dental pulp stem cells combined with polyglycolic acid contribute to periodontal regeneration.

Key words: materials, stem cells, bone, tissue engineering, gingiva, periodontium, bone defect, tissue regeneration

CLC Number:

Zhou Pengfei, Lin Jing, Chen Yuying, Lin Minkui. Canine dental pulp stem cells-polyglycolic acid scaffold complex for canine periodontal tissue defect[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5526-5531.

Figures/Tables 5

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