Icariin deproteinized inorganic bovine bone composite and deproteinized inorganic bovine bone material in repairing mandibular defects

doi:10.3969/j.issn.2095-4344.2878

Abstract

Abstract:

BACKGROUND: Deproteinized inorganic bovine bone is similar to human bone in structure. It has been widely used in oral bone regeneration surgery, but it lacks osteogenic induction ability. In recent years, it has been found that icariin has the effect on preventing and treating osteoporosis.

OBJECTIVE: To observe the effect of icariin deproteinized inorganic bovine bone composite in the repair of mandibular defects.

METHODS: MC3T3-E1 mouse osteoblasts were inoculated on icariin deproteinized inorganic bovine bone composite (observation group) and deproteinized inorganic bovine bone material (control group) respectively. After 7 days of culture, the survival of the cells on the surface of the material was observed by live-dead cell staining. The adhesion of the cells on the surface of the material was observed by scanning electron microscopy. After 5 and 10 days of culture, the alkaline phosphatase activity of the cells was detected. A 13 mm×6 mm×4 mm full-thickness defect of mandible was made in 30 New Zealand white rabbits. The experimental side (right) was implanted with icariin deproteinized inorganic bovine bone composite, and the control side (left) was implanted with deproteinized inorganic bovine bone material. The bilateral mandible tissues were obtained at 4, 8, and 12 weeks after the operation. The cone beam CT detection, histological observation and modified Gomori staining were performed respectively. The experiment was approved by the Ethics Committee of Experimental Animal Center of Mudanjiang Medical College.

RESULTS AND CONCLUSION: (1) Live-dead cell staining demonstrated that the osteoblasts on the surface of the two groups grew well. (2) Scanning electron microscopy showed that osteoblasts adhered to the surfaces of the two materials. The cells on the surface of observation group had more adhesion and more uniform distribution than those in the control group. (3) The alkaline phosphatase activity of observation group cultured for 10 days was higher than that of control group (P < 0.05). (4) Cone-beam CT showed that the bone defect on the experimental side healed at 12 weeks after operation, while the bone defect on the control side could be seen at 12 weeks after operation. (5) Histological observation showed that at 12 weeks after operation, a large number of mature bone tissue could be seen in the defect area on the experimental side, and only a small amount of residual material and adipose tissue could be seen. On the control side, although the material was partially degraded and more new bone tissue could be seen, but the bone maturity was lower than that on the experimental side. (6) Modified Gomori staining showed that at 12 weeks after operation, a large number of new bone tissue with higher maturity could be seen on the experimental side, and more mature bone tissue could be seen on the control side, but the bone mass and maturity were not as good as those on the experimental side. (7) Results confirmed that compared with deproteinized inorganic bovine bone material, icariin deproteinized inorganic bovine bone composite can promote the repair of mandibular defects.

Key words: bone, material, mandible, deproteinized inorganic bovine bone, icariin, bone defect, bone regeneration

CLC Number:

Dong Wenjie, Zhang Shiyang, Zhao Lei, Wang Yukun. Icariin deproteinized inorganic bovine bone composite and deproteinized inorganic bovine bone material in repairing mandibular defects[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5467-5472.

Figures/Tables 7

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