Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (10): 1495-1499.doi: 10.3969/j.issn.2095-4344.3067

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Rabbit skull defects repaired by the hydroxyapatite/geltin scaffold combined with bone marrow mesenchymal stem cells and umbilical vein endothelial cells

Fan Haixia1, Tan Qingkun1, Wang Hong2, Cheng Huanzhi1, Liu Xue1, Ching-chang Ko3, Geng Haixia1    

  1. 1Jining Medical University, Jining 272067, Shandong Province, China; 2Weifang Medical University, Weifang 261053, Shandong Province, China; 3School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill 27616, NC, USA
  • Received:2020-03-27 Revised:2020-04-01 Accepted:2020-05-09 Online:2021-04-08 Published:2020-12-17
  • Contact: Geng Haixia, Professor, Jining Medical University, Jining 272067, Shandong Province, China
  • About author:Fan Haixia, MD, Associate professor, Jining Medical University, Jining 272067, Shandong Province, China
  • Supported by:
    Shandong Natural Science Foundation, No. ZR2017LH044; Natural Science Foundation of Jining Medical University, No. 2016-09; College Students’ Innvovative Training of Jining Medical University, No. 201910443003 

Abstract: BACKGROUND: Hydroxyapatite-geltin composite has good biocompatibility and osteoinductivity, and can used be as tissue engineering scaffold to repair bone defects.
OBJECTIVE: To observe the effect of three-dimensional (3D) printed hydroxyapatite/gelatin scaffold combined with bone marrow mesenchymal stem cells and umbilical vein endothelial cells in repairing rabbit skull defects.
METHODS: Nine male New Zealand white rabbits were taken to establish a skull defect model with a diameter of approximately 0.8 cm, and randomly divided into three groups: blank group: no any treatment; control group: only 3D printed hydroxyapatite-geltin scaffold; experimental group: 3D printed hydroxyapatite-geltin scaffold and bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells complex group, with three rabbits in each group. At 8 weeks after the operation, CT scan of the skull pyramid beam and histological observation of the skull defect were performed on the white rabbits of each group. Animal experiments were approved by the Ethics Committee of Jining Medical College.
RESULTS AND CONCLUSION: (1) CT scan of pyramidal tract: Blank group showed obvious bone defects, and the defect area was slightly radiopaque with the edges of the surrounding normal bone tissue. Control group showed some new bone formation, which was discontinuous and inconsistent with the surrounding bone tissue. Experimental group showed that the new bone tissue was linear and continuous; the thickness was thin; and the defect area merged with the adjacent bone tissue edge. (2) Histological observation: Hematoxylin-eosin staining and Masson trichrome staining showed that fibrous connective tissue formation and a small amount of free bone cells were seen in the defect area of the blank group. A small amount of bone formation was seen in the control group. Bone matrix was deposited at the edge of the material, replacing the material to form small bone trabeculae. The material space of the experimental group was gradually replaced by new bone, and the defect area was filled with new bone and trabecular bone structure-like tissue. (3) The results show that the 3D bionic printing hydroxyapatite-geltin scaffold combined with bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells can effectively promote the growth of bone tissue and accelerate the repair of bone defects. 

Key words: stem cells">, bone">, bone regeneration">, 3D printing">, tissue engineering bone">, skull defect">, bone marrow mesenchymal stem cells">, umbilical vein endothelial cells

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