Porous beta-tricalcium phosphate-polypyrrole-biotin-icariin composite scaffold promotes recruitment of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2347

Abstract

Abstract:

BACKGROUND: Icaritin, as an osteogenic inducible active substance, has been widely loaded in orthopedic scaffold materials. However, in most of the existing studies, it is mainly placed in the pores of the scaffold directly in the form of polymerized microspheres, which makes it difficult to release icaritin in step with scaffold degradation, and the local effective utilization rate is low.

OBJECTIVE: To prepare porous β-tricalcium phosphate-polypyrrole-biotin-icariin microspheres composite scaffolds, and preliminarily explore the biological characteristics of coculture of the scaffolds and bone marrow mesenchymal stem cells.

METHODS: FeCl₃ was used as oxidant, and polypyrrole was synthesized by oxidative chemical synthesis. Polypyrrole, biotin and β-tricalcium phosphate were mixed and electrochemical synthesis was carried out. After that, the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold was prepared by three-dimensional printing technology. Icariin-biotin-polylactic acid microspheres were prepared by HDDD reaction and loaded on the scaffold above. The ordinary porous β-tricalcium phosphate scaffold prepared by three-dimensional printing was set as the control group and loaded with icaritin-polylactic acid microspheres. The compressive strength, porosity, drug loading, drug binding force and drug sustained release performance of the two groups of scaffolds were detected and compared, and the curve of icaritin release was drawn. The biological effects of the scaffolds on bone marrow mesenchymal stem cells were observed by scanning electron microscopy.

RESULTS AND CONCLUSION: The drug loading capacity, drug binding force and drug sustained release performance of the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold were significantly better than those in the control group (P < 0.05). The number of bone marrow mesenchymal stem cells growing on the surface of the scaffold under scanning electron microscopy was significantly higher than that of the control group (P < 0.05). There was no significant difference in the compressive strength and porosity of the scaffolds between the two groups (P > 0.05). The results confirmed that compared with the traditional sustained-release scaffolds, the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold further improves the drug loading capacity and sustained-release performance, and has good mechanical strength. Simultaneously, it may have a better role in recruiting bone marrow mesenchymal stem cells to participate in the bone repair around the scaffolds.

Key words:

stem cells">, , bone marrow mesenchymal stem cells">, , tricalcium phosphate">, , polypyrrole-biotin">, , icariin, scaffolds">, , bone">, , microspheres

CLC Number:

Liu Xin, Du Bin, Sun Guangquan, Cao Jinxing, Jiang Xiaohong. Porous beta-tricalcium phosphate-polypyrrole-biotin-icariin composite scaffold promotes recruitment of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5532-5537.

Figures/Tables 8

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