3D-printed icariin/decalcified bone matrix material promotes the repair of femoral condyle defects in rabbits

doi:10.3969/j.issn.2095-4344.2313

Abstract

Abstract:

BACKGROUND: Icariin can promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, and has good osteogenic properties. Decalcified bone matrix material exhibits good bone conduction and bone induction. It can be used in bone repair alone or in combination with other materials.

OBJECTIVE: To investigate the sustained-release performance, cytocompatibility and osteogenesis of 3D-printed icariin/decalcified bone matrix material.

METHODS: Icariin/decalcified bone matrix materials and decalcified bone matrix materials were prepared by 3D printing technology. The in vitro sustained-release performance of the icariin/decalcified bone matrix material was detected. Bone marrow mesenchymal stem cells were inoculated on the surfaces of the two materials respectively. The cells cultured separately were used as the control. At the designated time points, the live/dead staining and MTT assay were performed, and alkaline phosphatase activity and osteocalcin content were determined. Thirty New Zealand rabbit models of femoral condyle defect were prepared and then divided into three groups. In the control group, no implant was used. In the simple decalcified bone matrix group, decalcified bone matrix and allogeneic rabbit bone marrow mesenchymal stem cells composite were implanted. In the icariin/decalcified bone matrix group, icariin/decalcified bone matrix and allogeneic rabbit bone marrow mesenchymal stem cells were implanted. At 4 and 12 weeks after surgery, micro-CT and histological and mechanical properties evaluation were performed.

RESULTS AND CONCLUSION: (1) 3D-printed icariin/decalcified bone matrix material exhibited sustained-release performance. At 28 days, the release of icariin was (54.9±7.9)% of total release amount. (2) The live/dead staining revealed that the number of cells on the material surface in simple decalcified bone matrix and icariin/decalcified bone matrix groups were decreased after 1 day of inoculation and significantly increased with the prolongation of culture time. At 7 days, cells grew well, and the cells on the surface of icariin/decalcified bone matrix material were more evenly distributed and increased in number. (3) MTT test showed that cells in the icariin/decalcified bone matrix group proliferated faster after 7, 10, and 14 days of culture than those in the other two groups (P < 0.05). (4) The alkaline phosphatase activity and osteocalcin content in the icariin/decalcified bone matrix group were higher than those in the other two groups after 7, 10, and 14 days of culture (P < 0.05). (5) At 12 weeks after surgery, micro-CT revealed that a large amount of bone trabeculae were observed in both simple decalcified bone matrix and icariin/decalcified bone matrix groups. The number of bone trabeculae was higher, bone trabeculae were thicker, and defect cavity was smaller in the icariin/decalcified bone matrix group than those in the simple decalcified bone matrix group. (6) At 12 weeks after surgery, histological results revealed that a large number of bone tissue was formed in both simple decalcified bone matrix and icariin/decalcified bone matrix groups, but the amount of newly formed bone was higher in the latter group. (7) The compressive strength of bone defects in the icariin/decalcified bone matrix group was higher than that in the other two groups (P < 0.05). (8) These results suggest that 3D-printed icariin/decalcified bone matrix material exhibits good sustained-release performance, cytocompatibility, and osteogenesis.

Key words:

bone defect">, , icariin, decalcified bone matrix">, , bone regeneration">, , bone marrow mesenchymal stem cells">, , osteocalcin">, , alkaline phosphatase

CLC Number:

Zhang Huxiong, Li Wei, Yang Wupeng, New Suyaratu. 3D-printed icariin/decalcified bone matrix material promotes the repair of femoral condyle defects in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(34): 5461-5466.

Figures/Tables 10

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