Improving angiogenesis by collagen sponge carrying human adipose-derived stem cells

doi:10.3969/j.issn.2095-4344.2017.22.015

Abstract

Abstract:

BACKGROUND: Studies have found that adipose-derived stem cells (ADSCs)/collagen complexes can promote the ADSCs differentiation and maturation into mature adipocytes and promote angiogenesis.

OBJECTIVE: To explore the biological properties of the ADSCs/collagen sponge composite material and to detect its effect on angiogenesis.

METHODS: (1) ADSCs were cultured on collagen sponge (experimental group) or cultured alone (control group). After 24 hours of culture, cell adhesive rate of ADSCs was determined with flow cytometry. After 2, 4, 6 days of culture, cell proliferation and level of vascular endothelial growth factor (VEGF) in the culture medium were detected. (2) Chick embryo chorioallantoic membranes were exposed and incubated for 7 days and then divided into four groups: 0.2 mL of sterile PBS was added in the blank group, 0.2 mL of 2×10⁸/L passage 3 ADSCs suspension was added in the ADSCs group, collagen sponge was added in the collagen sponge group, and collagen sponge with 0.2 mL of 2×10⁸/L passage 3 ADSCs suspension was added in the composite group. After 7 days of incubation, the microvessel count around the chorioallantoic membrane was measured.

RESULTS AND CONCLUSION: (1) The cell adhesive rate of ADSCs to collagen sponge reached to (93.04±0.67)%. (2) The absorbance value (at 6 days of culture) and level of VEGF (at 4 and 6 days of culture) in the experimental group were significantly higher than those in the control group (P < 0.01 or P < 0.05). (3) Compared with the blank group, the number of microvessels was significantly higher in the ADSCs, collagen sponge and composite groups (P < 0.05). Moreover, higher amount of microvessels were found in the composite group than the ADSCs and collagen sponge groups (P < 0.05). To conclude, ADSCs can adhere well to the collagen sponge with good biocompatibility and their combined use can improve angiogenesis further by enhancing cell proliferation and VEGF secretion of ADSCs.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen, Stem Cells, Tissue Engineering

CLC Number:

R318

Xue Jing-xian, Chen Xu, Xu Yan-lei, Ren Wei-ye, Li Yan, Zhang Yu-xuan, Yao Chang.

Improving angiogenesis by collagen sponge carrying human adipose-derived stem cells

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(22): 3531-3535.

Figures/Tables 5

References

[1]Cunningham BL,Lokeh A,Gutowski KA.Saline-filled breast implant safty and efficacy：a multicenter retrospective review.Plast Reconstr Surg.2000;105(6):2143-2149．

[2]戚可名.女性整形美容外科学[M].北京:人民军医出版,2001: 673-674．

[3]Ryuto M,Ono M,lzumi H,et al．Induction of vascular endothelial growth factor by tumor necrosis factor alpha in human glioma cells.Possible roles of SP-1.J Biol Chem. 1996;27(45):28220-28228．

[4]Hong S,Traktuev J,March KL,et al.Therapeutic potential of adipose-derived stem ce11s in vascsular growth and tissue repairs.Curr Opin Organ Transplant.2010;15(1): 86-91

[5]杨超,邢新,徐达圆,等.脂肪干细胞－透明质酸复合物促进放射性复合损伤创面愈合的初步研究[J].中国修复重建外科杂志, 2011, 25(12):1499-1501.

[6]Hattori H,Masuoka K,Sato M,et al.Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold.J Biomed Mater Res B Appl Biomater. 2006;76(1):230-239.

[7]张云松,高建华,鲁峰,等.I型胶原支架材料与人脂肪干细胞体外生物相容性研究[J].南方医科大学学报,2007,27(2):223-225

[8]陆伟,金岩,张勇杰,等.脂肪组织来源干细胞复合胶原构建组织工程皮肤修复皮肤缺损[J].实用口腔医学杂志, 2007,23(2): 161-164.

[9]Wosnitza M,Hemmrich K,Pallua N.Plasticity of human adipose stem cells to perform adipogenic and endothelial differentiation.Differentiation.2007;75(1):12-23.

[10]Ribatti D.Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis.Int Rev Cell Mol Biol.2008;270: 181-224.

[11]唐昱,盛国太,钟志英.丹红注射液促进鸡胚绒毛尿囊膜血管生成的实验研究[J].中西医结合心脑血管病杂志, 2010,8(3): 3110-3111

[12]Sun X,Altalhi W,Nunes SS.Vascularization strategies of engineered tissues and their application in cardiac regeneration.Adv Drug Deliv Rev.2016;96:183-194.

[13]Yao C,Roderfeld M,Rath T,et al.The impact of proteinase-induced matrix degradation on the release of VEGF from heparinized collagen matrices.Biomaterials. 2006;27(8): 1608-1616.

[14]Steffens GC,Yao C,Prevel P,et al.Modulation of angiogenic potential of collagen matrices by covalent incorporation of heparin and loadig with vascular endothelial growth factor.Tissue Eng.2004;10(9-10):1502-1509.

[15]Koch S,Yao C,Grieb G,et al.Enhancing angiogenesis in collagen matrices by covalent incorporation of VEGF.Mater Sci: Mater Med.2006;17:735-741.

[16]Lindroos B,Suuronen R,Miettinen S.The potential of adipose stem cells in regenerative medicine.Stem Cell Rev.2011;7(2): 269-291.

[17]李晓童,支运霞,张喜,等.脂肪源性干细胞与胶原海绵支架的相容性[J].解剖学杂志,2012,35(6):741-744,769.

[18]徐永飞,张建文,暴志国,等.脂肪来源干细胞复合不同生物支架在裸鼠皮下的促血管化作用[J].郑州大学学报(医学版), 2013, 39(6): 773-776.

[19]许颖溦,刘林嶓,徐永飞,等.脂肪干细胞复合Ⅰ型胶原对裸鼠移植组织生长的影响[J].郑州大学学报(医学版), 2014,40(2): 255-257.

[20]乐音子,陈灵,任伟业,等.胶原海绵对创面愈合的影响[J].中国基层医药,2014,21(13):1954-1956.

[21]魏庆,任伟业,张晶,等.胶原海绵在慢性下肢溃疡中应用临床疗效观察[J].安徽医药,2012,16(7):949-951.

[22]陈运,邵大畏,任伟业,等.胶原海绵填充甲状腺手术残腔与止血疗效临床观察[J].中国医药指南,2012,10(5):79-80.

[23]邵大畏,陈运,任伟业,等.胶原海绵填充乳腺缺损20例临床观察[J].中国医药指南,2012,10(2):85-86.

[24]于志永,付勤,张涛,等.脂肪间充质干细胞复合壳聚糖-胶原-硫酸软骨素多孔支架体外成软骨能力的实验研究[J].中国骨质疏松杂志,2009,15(3):165-170.

[25]杨震,李波,周焯家,等.人脂肪干细胞体外培养及细胞载体复合物诱导培养[J].中国老年学杂志,2014,34(18):5187-5190.

[26][26] Han B,Wang HC,Li H,et al.Nucleus pulposus mesenchymal stem cells in acidic conditions mimicking degenerative intervertebral discs give better performance than adipose tissue-derived mesenchymal stem cells.Cells Tissues Organs. 2014;199(5-6):342-352.

[27]李琳,姚昶,朱永康,等.胶原海绵促进人脐静脉血管内皮细胞生长的体外实验研究[J].临床和实验医学杂志, 2014,13(18): 1494-1496.

[28]周勇,任伟业,邵大畏,等.胶原海绵促进创面止血与血管新生的实验研究[J].湖南中医杂志,2012,28(3):140-141,165.