BACKGROUND: Recent research has shown that the proper concentration of aspirin can increase the proliferation and osteogenic ability of MG-63 cells.
OBJECTIVE: To explore the effects of different concentrations of aspirin on osteoblast proliferation on the implant-cell interface under fluid shear stress.
METHODS: (1) MG-63 cells were cultured in low-glucose DMEM containing 10% fetal bovine serum and different concentrations of aspirin (0, 0.023, 0.046, 0.0625, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0 mmol/L) for 1-7 days. Then cell proliferation was detected using MTS method. (2) MG-63 cells were cultured on three different surfaces: glass slide, PT titanium surface and SLA titanium surface. After 3 days of culture with aspirin at a concentration of 0 or 0.5 mmol/L, the cells were subjected to fluid shear stress. MTS test was applied to estimate the proliferation of MG-63 cells at 0, 0.5, 1, 2, 4 hours after stress application.
RESULTS AND CONCLUSION: (1) After 1-3 days of culture, 0.023, 0.046, 0.5 mmol/L aspirin promoted the proliferation of MG-63 cells, while after 1-7 days of culture, 1, 2, 4 mmol/L aspirin inhibited the proliferation of MG-63 cells. (2) Under the fluid shear stress, aspirin showed significant effects on the cell proliferation as confirmed by one-way analysis of variance (
F=8.349,
P=0.004), and 0.5 mmol/L aspirin inhibited the cellular proliferation of MG-63 cells. However, surface modification and stress loading time showed no significant effects on the cell proliferation (
F=2.826,
P=0.064;
F=0.893,
P=0.406). (3) Under the fluid shear stress, surface modification showed no significant effect on the cell proliferation of MG-63 cells cultured with 0.5 mmol/L aspirin (
F=1.803,
P=0.171). Under the fluid shear stress, 0.5 mmol/L aspirin significantly inhibited the proliferation of MG-63 cells on the glass slide (
P=0.003), while PT and SLA titanium surfaces showed on inhibitory effect on the cell proliferation (
P=0.891,
P=0.051). The present results demonstrate that the cell proliferation of MG-63 is related with aspirin in a concentration-dependent manner. In addition, different titanium surfaces may decrease the sensitivity of MG-63 cell proliferation to aspirin.