Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (22): 3555-3560.doi: 10.3969/j.issn.2095-4344.2017.22.019

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Aspirin effects on MG-63 cell proliferation on different modified titanium surfaces under fluid shear stress

Liang Zhong-lang1, Liu Chang-hong2, Li Shi-yi1, Yang Xiao-yu1 
  

  1. 1Stomatological Hospital, Southern Medical University, Guangzhou 510260, Guangdong Province, China; 2Guangdong Xieda Stomatological Hospital, Guangzhou 510399, Guangdong Province, China
  • Received:2017-06-19 Online:2017-08-08 Published:2017-09-01
  • Contact: Yang Xiao-yu, M.D., Chief physician, Master’s supervisor, Stomatological Hospital, Southern Medical University, Guangzhou 510260, Guangdong Province, China
  • About author:Liang Zhong-lang, Master, Stomatological Hospital, Southern Medical University, Guangzhou 510260, Guangdong Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 30500569

Abstract:

BACKGROUND: Recent research has shown that the proper concentration of aspirin can increase the proliferation and osteogenic ability of MG-63 cells.
OBJECTIVE: To explore the effects of different concentrations of aspirin on osteoblast proliferation on the implant-cell interface under fluid shear stress.
METHODS: (1) MG-63 cells were cultured in low-glucose DMEM containing 10% fetal bovine serum and different concentrations of aspirin (0, 0.023, 0.046, 0.0625, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0 mmol/L) for 1-7 days. Then cell proliferation was detected using MTS method. (2) MG-63 cells were cultured on three different surfaces: glass slide, PT titanium surface and SLA titanium surface. After 3 days of culture with aspirin at a concentration of 0 or 0.5 mmol/L, the cells were subjected to fluid shear stress. MTS test was applied to estimate the proliferation of MG-63 cells at 0, 0.5, 1, 2, 4 hours after stress application.
RESULTS AND CONCLUSION: (1) After 1-3 days of culture, 0.023, 0.046, 0.5 mmol/L aspirin promoted the proliferation of MG-63 cells, while after 1-7 days of culture, 1, 2, 4 mmol/L aspirin inhibited the proliferation of MG-63 cells. (2) Under the fluid shear stress, aspirin showed significant effects on the cell proliferation as confirmed by one-way analysis of variance (F=8.349, P=0.004), and 0.5 mmol/L aspirin inhibited the cellular proliferation of MG-63 cells. However, surface modification and stress loading time showed no significant effects on the cell proliferation (F=2.826, P=0.064; F=0.893, P=0.406). (3) Under the fluid shear stress, surface modification showed no significant effect on the cell proliferation of MG-63 cells cultured with 0.5 mmol/L aspirin (F=1.803, P=0.171). Under the fluid shear stress, 0.5 mmol/L aspirin significantly inhibited the proliferation of MG-63 cells on the glass slide (P=0.003), while PT and SLA titanium surfaces showed on inhibitory effect on the cell proliferation (P=0.891, P=0.051). The present results demonstrate that the cell proliferation of MG-63 is related with aspirin in a concentration-dependent manner. In addition, different titanium surfaces may decrease the sensitivity of MG-63 cell proliferation to aspirin.

Key words: Aspirin, Biomechanics, Titanium, Osteoblasts, Cell Proliferation, Tissue Engineering

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