Expression of nuclear factor-kappa B in rat models of periapical periodontitis

doi:10.3969/j.issn.2095-4344.2016.49.020

Abstract

Abstract:

BACKGROUND: mRNA expression level of nuclear factor-kappa B (NF-?B) in periapical periodontitis has been shown to be significantly higher than that in normal periapical tissue.
OBJECTIVE: To observe the expression of NF-?B in rats with periapical periodontitis.
METHODS: Twenty male Sprague-Dawley rats aged 6 weeks were enrolled to establish apical periodontitis model at the right mandibular first motar (experimental group), and the left mandibular first motar as normal control group. Five rats were randomly selected at postoperative 1, 2, 3, and 4 weeks to make frozen sections of the mandibular tissue. The morphology of the periapical tissue was observed through hematoxylin-eosin staining, and the expression level and distribution of NF-?B were detected by immunohistochemical staining.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed that the apical periodontal tissue of the mandibular first molar at the right side had local inflammatory response and spread to peripheral tissues; while there was no pathological changes in the control group. (2) Immunohistochemistry results revealed that NF-?B expression increased from the 1st week to 2nd week after surgery, and then went down at the 3rd week and reached the lowest point at the 4th week; moreover, the expression level at each time exhibited significant differences (P < 0.05). The amount of NF-?B-positive cells in the experimental group was significantly higher than that in the control group (P < 0.05). (3) These findings suggest that the expression level of NF-?B holds close correlation with the process of periapical periodontitis indicating that NF-?B may participate in the inflammatory reaction of periapical periodontitis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: NF-kappa B, Tooth, Periodontitis, Tissue Engineering

CLC Number:

R318

Ye Dan-dan, Wang Ting-ting, Dong Ming, Jin Hai-wei, Niu Wei-dong. Expression of nuclear factor-kappa B in rat models of periapical periodontitis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7431-7436.

Figures/Tables 2

References

[1] Kakehashi S, Stanley H, Fitzgerald R. The effect of surgical exposures of d-entalpulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol.1965;20(3): 340-349.
[2] Karin M, Lin A．NF-kappa B at the crossroads of life and death．Nat Immunol. 2002;3(3):221-227．
[3] Zhang L,Shao L,Creighton CJ,et al.Function of phosphorylation of NF-kB p65 ser536 in prostate cancer oncogenesis. Oncotarget. 2015;6(8): 6281-6294.
[4] Zhang L,Shao L,Creighton CJ,et al.Function of phosphorylation of NF-kB p65 ser536 in Zhao W,Sun Z,Wang S,et al.Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB.Inflammation. 2015; 38(4):1700-1706.
[5] Kowalewsk C,Kurpios-Pieca D,Rahden-Staron L,et alThe potential role of NF-kB activation and synthesis of pro-inflammatory mediators in the pathogenesis of allergic contact dermatitis induced by thiuram.Clinical and Translational Allergy.2015;5(Suppl 1):O22.
[6] Wullaert A. Role of NF-B activation in intestinal immune homeostasi. Intern J Med Microbiol.2010; 300(1) :49-56．
[7] Schimmer AD, Welsh K, Pinlla C, et al. Small molecule antagonists of apoptosis suppressor XIAP exhibit broad antitumor activity. Cancer Cell.2004;5(1):25-35.
[8] Clohisy JC, Roy BC, Biondo C, et al. Direct inhibition of NF- kappa B blocks bone erosion associated with inflammatory arthritis. Jmmunol. 2003;171(10): 5547-5553.
[9] Boyce BF, Yao Z, Xing L. Functions of nuclear factor kappaB in bone.Ann N Y Acad Sci.2010;1192: 367-375.
[10] Wullaert A, Bonnet MC, Pasparakis M.NF-kappaB in the regulation of epithelial homeostasis and inflammation.Cell Res.2011;21(1):146-158.
[11] Roser VT, Javier RU, Lorena DL,et al. NF-κB directly mediates epigenetic deregulation of common microRNAs in Epstein-Barr virus-mediated transformation of B-cells and in lymphomas. Nucleic Acids Res. 2014;42(17):11025-11039.
[12] Crabtree M, Pileggi R, Bhattacharyya I, et al. RAGE mRNA Expression and Its Correlation with Nuclear Factor Kappa Beta mRNA Expression in Inflamed Human Periradicular Tissues. J Endod. 2008;34(6): 689-692.
[13] Arabaci T, Cicek Y, Canakci V, et al. Immunohistochemical and Stereologic Analysis of NF-κB Activation in Chronic Periodontitis. Eur J Dent. 2010,(4):454-461.
[14] 袁洪峰, 贺翔鸽.核因子kB与眼科疾病的研究进展[J].国际眼科杂志,2008,8(1):132-136.
[15] Takashiba S, Van byke TE, Amar S.Inhibition of nuclear factor kappa B subnit P65mRNA acuumulation in lipopolysaccharide-stimulated human monocytic cells treated with sodium Salicylate. Oral Microbiol Immunol.1996;11:420.
[16] Mukaida N, Ishikawa Y, Ikeda N, et al. Increase of chemokine levels in sputum precedes exacerbation of acute asthma attacks. J Leukoc Biol.1996;59:145.
[17] Xiao L, Zhu LX, Yang SS, et al. Different Correlation of Sphingosine-1-Phosphate Receptor 1 with Receptor Activator of Nuclear Factor Kappa B Ligand and Regulatory T Cells in Rat Periapical Lesions.J ENDODONT. 2015;41(4):479-486.
[18] 陈兴兴,谷苗,吕海鹏,等不同方法建立实验性狗根尖周炎模型的比较[J].口腔医学研究[J],2009,25(4):416-419.
[19] Chuang FH, Tsai CC, Chen JH, et al. Long-term sequential receptor activator of NF-kappa B ligand (RANKL) and osteoprotegrin (OPG) expression in lipopolysaccharide-induced rat periapical lesions.J ORAL PATHOL MED. 2012;41(2):186-193.
[20] Sen R, Baltimore D. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.Cell. 1986;46(5):705-716.
[21] Baldwin AS Jr. TheNF-kB and IkB Proteins; new diseoveries and insights. Annu Rev Immunol. 1996; 14:649-683.
[22] Perkins ND. The Rel P/NF-κB family : friend and foe . Trends Biochem Sci, 2000,25(9):434-440.
[23] Choi KC, Lee YH, Jung MG, et al.Gallic acid suppresses lipopolysaccha ride-induced nuclear factor-kappaB signaling by preventing RelA acetylation in A549 lung cancer cells.Mol Cancer Res,2009,7(12): 2011-2021.
[24] Sung B, Pandey MK, Ahn KS, et al. Anacardic acid( 6-nonadecyl salicylicacid) , aninhibitor of histone acetyltransferase,suppresses expression of nuclear factor-kappaB-regulated gene products involved in cell survival,proliferation,invasion,and inflammation through inhibition of the inhibitory subunit of nuclear factor-kappaBalpha kinase,leading to potentiationof apoptosis.Blood.2008;111(10):4880-4891.
[25] Yang XD, Tajkhorshid E, Chen LF.Functional interplay between acetylation and methylation of the RelA subunit of NF-kappaB.Mol Cel Biol.2010;30(9) : 2170-2180.
[26] Lauzurica P, Martinez MS, Marazuela M, et al. Pyrrolidine dithiocarbamate protects mice from lethal shock induced by LPS or TNF-alpha. Eur J Immunol. 1999;29(6):1890-1900.
[27] Muià C, Mazzon E, Maiere D, et al.Pyrrolidine dithiocarbamate reduced experimental periodontitis. Eur J Pharmacol. 2006;539(3):205-210.