Effect of constipation induced by diphenoxylate on intestinal flora in a rat

doi:10.3969/j.issn.2095-4344.2016.49.016

Abstract

Abstract:

BACKGROUND: In the previous studies, the overall index in constipation models, such as fecal output in 24 hours and intestinal tension are usually measured. However, the changes of intestinal flora are rarely involved.
OBJECTIVE: To explore the effect of constipation on intestinal tension, pathology and microecology in the rat models of diphenoxylate-induced constipation.
METHODS: Twenty SPF Wistar rats were randomly divided into blank control and model groups, followed by given 10 mg/kg diphenoxylate and distilled water by gavage once daily for 20 days, respectively.
RESULTS AND CONCLUSION: Twenty days later, compared with the blank control group, the frequency of ileum contraction was decreased, 24-hour stool decreased, the ratio of bacteria group in the cecum changed, and structure of the colon was seriously damaged in the model group. These results suggest that constipation results in the intestinal flora disturbance remarkablely.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Constipation, Diphenoxylate, Enterobacteriaceae, Tissue Engineering

CLC Number:

R318

Li Gen-lin, Zhang Yan-yu, Li Han-bing. Effect of constipation induced by diphenoxylate on intestinal flora in a rat[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7404-7410.

Figures/Tables 4

References

[1] 谢培,周永学.大鼠功能性便秘模型建立方法的研究[J].陕西中医,2014,35(3):377-379.
[2] 赵兵,唐学贵,吴至久,等.复方地芬诺酯建立便秘模型与AQP8的相关性研究[J].中医临床研究,2015(18): 107-110.
[3] Jeffery IB, O'Toole PW, Öhman L, et al. An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota. Gut. 2012;61(7): 997-1006.
[4] Bested AC, Logan AC, Selhub EM. Intestinal microbiota, probiotics and mental health: from Metchnikoff to modern advances: Part II - contemporary contextual research. Gut Pathog. 2013;5(1):3.
[5] Suzuki TA, Worobey M. Geographical variation of human gut microbial composition. Biol Lett. 2014; 10(2):20131037.
[6] 李寒冰,吴宿慧,张颜语,等.中药与肠道菌相互作用研究进展[J].中成药,2016,38(1):147-151.
[7] 郭慧玲,邵玉宇,孟和毕力格,等.肠道菌群与疾病关系的研究进展[J].微生物学通报,2015,42(2):400-410.
[8] Yu Z, Morrison M. Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol. 2004;70(8):4800-4806.
[9] Li T, Lu X, Yang X. Stachyose-enriched α-galacto-oligosaccharides regulate gut microbiota and relieve constipation in mice. J Agric Food Chem. 2013; 61(48):11825-11831.
[10] 胡勇,侯建勇,唐文凤.革兰氏染色实验教学探讨[J].实验室科学,2014,17(6):120-122.
[11] Li AJ, Yang SF, Li XY, et al. Microbial population dynamics during aerobic sludge granulation at different organic loading rates. Water Res. 2008;42(13): 3552-3560.
[12] 张海丽.牛肠道病毒的分离鉴定及HLJ-3531株感染性分子克隆的建立[D].哈尔滨:东北农业大学,2015.
[13] Wallace JL, Keenan CM. An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis. Am J Physiol. 1990;258(4 Pt 1): G527-534.
[14] 郑倩.便秘动物模型的研究进展[J].临床消化病杂志,2012, 24(3):189-191.
[15] Raes J. The gut microbiome - a new target for understanding, diagnosing and treating disease. Arch Public Health. 2014; 72(Suppl 1): K3.
[16] Koeth RA, Wang Z, Levison BS, et al. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat Med. 2013;19(5): 576-585.
[17] Goodman AL, Gordon JI. Our unindicted coconspirators: human metabolism from a microbial perspective. Cell Metab. 2010;12(2):111-116.
[18] Zhu L, Liu W, Alkhouri R, et al. Structural changes in the gut microbiome of constipated patients. Physiol Genomics. 2014;46(18):679-686.
[19] Zoppi G, Cinquetti M, Luciano A, et al. The intestinal ecosystem in chronic functional constipation. Acta Paediatr. 1998;87(8):836-841.
[20] Kaiko GE, Ryu SH, Koues OI, et al. The Colonic Crypt Protects Stem Cells from Microbiota-Derived Metabolites. Cell. 2016;165(7):1708-1720.
[21] 白云.表达鼠源GLP-2嗜酸乳杆菌的构建及其对IBD模型小鼠的影响[D].长春:吉林农业大学,2015.