Histopathological features of the rat ileum after Shigella flexneri infection

doi:10.3969/j.issn.2095-4344.2016.49.017

Abstract

Abstract:

BACKGROUND: Bacillary dysentery is one of the most common enteric infectious diseases in summer and autumn that is mainly caused by Shigella.
OBJECTIVE: To analyze the pathological features of the rat ileum after Shigella flexneri infection.
METHODS: Totally 30 male Sprague-Dawley rats were enrolled and equally randomized into three groups. Rats in non-streptomycin and streptomycin groups were firstly treated with normal saline and 50 g/L streptomycin sulphate via gastric lavage, and then given gastric lavage of 9×108 CFU/mL Shigella flexneri. Rats were given no any treatment as control group. 14 days later, morphological changes of the rat ileum were observed through hematoxylin-eosin staining.
RESULTS AND CONCLUSION: (1) After Shigella flexneri infection, the rat distal ileum and rectum were characterized by mucosal edema and hemorrhage, neutrophilic granulocyte exudation, pseudomembrane formation and destruction of goblet cells with hemorrhage. There were obvious fibrin exudation on the necrotic mucosal surface that constituted the pseudomembrane with necrotic debris, leukocytes and erythrocytes. After mucosa defulvium, non-uniform and superficial ulcers were mainly visible at the mucosa. (2) The occurrence time of positive systems, temperature and body mass before and after Shigella flexneri infection showed no significant differences among rats (P > 0.05). (3) These results suggest that the rat distal ileum and rectum after Shigella flexneri infection present the characteristic morphological futures of bacillary dysentery; therefore, it can be used as the animal model to undergo in-depth research about the etiology, pathogenesis and treatment of bacillary dysentery.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Shigella dysenteriae, Colon, Pathology, Tissue Engineering

CLC Number:

R318

Luo Su-yi, Wang Xi-yun, Li Jin-tao. Histopathological features of the rat ileum after Shigella flexneri infection[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(49): 7411-7417.

Figures/Tables 3

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