Effect of miR-1231 on proliferation, apoptosis and invasion of colon cancer stem cells

doi:10.3969/j.issn.2095-4344.2016.45.009

Abstract

Abstract:

BACKGROUND: Previous studies have found that miR-1231 is down-regulated in colon cancer stem cells (CCSCs), but the effect of miR-1231 on CCSCs remains unclear.
OBJECTIVE: To explore the effect of miR-1231 on the proliferation, apoptosis and invasion of CCSCs (CD133⁺CD44⁺).
METHODS: CD133⁺CD44⁺ cells and CD133^-CD44^- cells were separated from SW1116 cells by immunomagnetic bead separation. The expression level of miR-1231 in CD133⁺CD44⁺ and CD133^-CD44^- cells was detected by qRT-PCR. miR-1231-overexpressing CD133⁺CD44⁺ cells were transfected with miR-1231 mimics or miR-control by lipofection transfection. The effects of miR-1231 on CD133⁺CD44⁺ cell proliferation, apoptosis and invasion were investigated by MTT, flow cytometry and Transwell assays, respectively. In addition, the expression levels of Ki67, Bax, Bcl-2, MMP-2 and MMP-9 protein in miR-1231-overexpressing CD133+CD44+ cells and control cells were detected by western blot.
RESULTS AND CONCLUSION: CD133⁺CD44⁺ and CD133^-CD44^- cells were obtained by the immunomagnetic bead separation. The expression level of miR-1231 in CD133⁺CD44⁺ cells was significantly lower than that in CD133^-CD44^- cells. miR-1231 suppressed CD133⁺CD44⁺ cell proliferation and invasion, but promoted the apoptosis in these cells. Western blot analysis showed that miR-1231-overexpressing CD133⁺CD44⁺ cells had obvious decreases in Ki67, Bcl-2, MMP-2 and MMP-9 protein expression and a significant increase in Bax protein expression compared with control cells. All these results further confirm that miR-1231 inhibits the proliferation and invasion but promotes the apoptosis in CD133⁺CD44⁺ cells. These findings suggest that miR-1231 can be a suppressor of CCSCs, which offers a novel potential therapeutic target for CCSCs and colon cancer.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Colonic Neoplasms, Neoplastic Stem Cells, MicroRNAs, Transfection, Cell Proliferation, Apoptosis, Tissue Engineering

CLC Number:

R394.2

Yan Li-kun, Li Wei, Yao Jian-feng, Mao Zhi-jun . Effect of miR-1231 on proliferation, apoptosis and invasion of colon cancer stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45): 6746-6752.

Figures/Tables 2

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