Different exercise intensities affect bone mineral density in type 2 diabetic rats

doi:10.3969/j.issn.2095-4344.2016.27.002

Abstract

Abstract:

BACKGROUND: More than 50% of patients with diabetes are accompanied by osteoporosis. Exercise is the main method to treat diabetes, but whether it has an impact on diabetes osteoporosis is unclear.
OBJECTIVE: To analyze the different exercise intensities and changes in bone mineral density in type 2 diabetic rat models.
METHODS: Forty rats were randomly divided into model group and low-, medium- and high-intensity exercise groups. All rats received intraperitoneal injection of streptozotocin to establish models of type 2 diabetes. Rats in the low-, medium- and high-intensity exercise groups did treadmill training at 10, 20, 30 m/min, 1 hour per day, 6 days as a cycle. They had a rest for 1 day after each cycle, for 8 consecutive cycles. Rats in the model group did not do any exercise.
RESULTS AND CONCLUSION: Compared with the model group, the weight of rats was decreased; blood glucose levels were reduced; osteocalcin and serum calcium levels and biomechanical indexes of femur increased; serum phosphorus and alkaline phosphatase decreased in the low-, medium- and high-intensity exercise groups. Bone mineral density increased in the medium- and high-intensity exercise groups. These findings confirmed that different exercise intensities have a certain influence on bone mineral density and bone metabolism in type 2 diabetic rat models. When using a method of treating movement, proper physical exercise can increase serum osteocalcin content, improve bone metabolism, prevent diabetic osteoporosis, and have a high clinical value.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Diabetes Mellitus, Osteoporosis, Tissue Engineering

CLC Number:

R318

Jiang Hong. Different exercise intensities affect bone mineral density in type 2 diabetic rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(27): 3963-3969.

Figures/Tables 3

2.1 造模成功动物数量及过程 20只大鼠全部进行结果分析数量，中途无脱落。 2.2 模型方法的改进及模型稳定性对大鼠高脂饲料喂养8周联合小剂量链脲佐菌素腹腔注射制备2型糖尿病大鼠模型，制备相对比较简单，省去了繁琐的程度，对于实验室的要求也相对较低。制备的2型糖尿病以测定血糖作为建立成功的标准，使得模型稳定性更强，更加符合临床实践的要求。 2.3 主要观察指标和次要观察指标 2.3.1 体质量 4组大鼠造模后体质量总体呈现上升趋势；低强度运动组、中强度运动组和高强度运动组大鼠体质量显著低于模型组(P < 0.05；表1，图1)。说明运动能控制大鼠的体质量，有益于控制机体血糖。 2.3.2 血糖及骨密度造模后8周测定4组大鼠血糖，与模型组相比，低强度运动组、中强度运动组以及高强度运动组大鼠血糖水平明显下降(P < 0.05)，且低强度运动组、中强度运动组以及高强度运动组大鼠血糖水平接近(P > 0.05；表2，图2)。与模型组相比，中、高强度运动组大鼠双侧股骨骨密度显著上升(P < 0.05)，但低强度运动组大鼠骨密度变化差异无显著性意义(P > 0.05；表2，图2)。提示对于2型糖尿病大鼠来说，适当的的运动能有效的控制机体血糖，提高大鼠骨密度水平。 2.3.3 骨代谢与模型组相比，低、中、高强度运动组大鼠骨钙素以及血钙水平显著上升(P < 0.05)，血磷和碱性磷酸酶指标显著下降(P < 0.05)；随着训练强度的增加大鼠骨密度代谢水平得到改善(表3，图3)。 2.3.4 生物力学指标与模型组相比，低强度运动组、中强度运动组以及高强度运动组大鼠股骨最大载荷、最大应力、模量以及能量显著增加(P < 0.05)；低、中、高强度运动组大鼠股骨最大应力、模量差异无显著性意义(P > 0.05)；大鼠最大载荷、最大应力随着运动强度增加出现上升趋势(表4，图4)。"

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