Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (38): 6184-6189.doi: 10.3969/j.issn.2095-4344.2015.38.021

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Injectable nano/chitosan/bone morphogenetic protein-2 induces periodontal tissue regeneration

Ba Ge-na1, 2, Chen Hua-rong1, 2, Li Ting1, 2, Xie Fu-qiang1, Yu Ling-hui3   

  1. 1Second Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China; 
    2School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; 
    3Department of Stomatology, Donggang Branch, First Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
  • Contact: Corresponding author: Xie Fu-qiang, Professor, Chief physician, Second Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China Yu Ling-hui, Associate professor, Chief physician, Department of Stomatology, Donggang Branch, First Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
  • About author:Ga Ge-na, Studying for master’s degree, Second Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China; School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China
  • Supported by:

    the Science and Technology Plan of Lanzhou City, No. 2011-2-50; the Science and Technology Plan of Gansu Province, No. 1011FKCA103

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Abstract:

BACKGROUND: Chitosan hydrogel has good biocompatibility, biodegradability and antibacterial property, which can promote tissue healing and induce bone formation. As a scaffold carrying growth factors, it can ensure the efficient and slow release of exogenous growth factors.
OBJECTIVE: To observe the effect of injectable nano/chitosan/bone morphogenetic protein-2 composite to promote periodontal tissue regeneration in rats.
METHODS: Fifty-four Wistar rats were randomized into three groups, and then chronic periodontitis model of the second molar was established. After modeling, injectable nano/chitosan/bone morphogenetic protein-2 composite was implanted into the periodontal tissue of the second molar in the experimental group; injectable nano/chitosan hydrogel was implanted in the control grouop; and nothing was implanted in the blank group. At 3, 6, 9 weeks after surgery, gingival bleeding index, probing depth, and tooth mobility were detected. X-ray and histopathological observations were carried out.
RESULTS AND CONCLUSION: At 9 weeks after surgery, the probing depth and tooth mobility were both lower in the experimental group than the other two groups (P < 0.05). In the experimental group, the alveolar bone height was restored at the root bifurcations, bone trabeculae were arranged densely and evenly, the newly formed periodontal ligament and alveolar bone tissues were dense and equally distributed in the bone defect area at 9 weeks after surgery, indicating a better restoration effect than the control and blank groups. These findings suggest that the injectable nano/chitosan/bone morphogenetic protein-2 composite has an anti-inflammatory role and can guide periodontal tissue regeneration.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Gels, Bone Morphogenetic Proteins

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