Preparation and performance detection of carbon fiber-polylactic acid-polyethylene glycol composite scaffold

doi:10.3969/j.issn.2095-4344.2015.21.017

Abstract

Abstract:

BACKGROUND: The mechanical performance and cytocompatibility of polylactic acid-polyethylene glycol (PLA-PEG) scaffold is poor via long-term experiments; therefore, other materials are added to the scaffold in order to improve its biological activity and mechanical properties.
OBJECTIVE: To investigate the preparation process of carbon fiber (CF)-PLA-PEG scaffold, and to test its performance.
METHODS: Modified CF-PLA-PEG composite scaffolds were prepared using solution injection-particle leaching method. Ultra-structure, porosity, water-absorbing quality, degradation rate and mechanical properties of CF-PLA-PEG composite scaffolds and PLA-PEG scaffolds were compared. Rat osteoblasts were co-cultured with these two kinds of scaffolds in vitro, and the cell adhesion rate was detected by precipitation method after 12 hours. Cell counting was detected using MTT method to determine the cell proliferation at culture days 1, 3, 5, 7, 9.
RESULTS AND CONCLUSION: The surface structure of PLA-PEG distributed uniformly, and the aperture was
(404.0±10.5) µm. The carbon fiber surface of modified CF-PLA-PEG composite scaffolds had a lot of longitudinal grooves, the surface structure also distributed uniformly, and the aperture was (433.0±3.0) µm. Difference in scaffold aperture was of significance between the two groups (P < 0.05). The porosity, water-absorbing quality, elasticity modulus, compressive strength, degradation rate, cell adhesion rate and cell growth rate of CF-PLA-PEG were better than those of PLA-PEG (P < 0.05). These results show that the CF-PLA-PEG scaffolds have good mechanical property and cell compatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Polyethylene Glycols, Biomechanics

CLC Number:

R318

Zhou Chang-yan, Zhou Qing-huan, Bian Jing, Chen Ke, Chen Wen. Preparation and performance detection of carbon fiber-polylactic acid-polyethylene glycol composite scaffold [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3371-3376.

Figures/Tables 4

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