Chitosan microspheres loading whole cell protein antigen of Helicobacter pylori: preparation and in vitro release characteristics

doi:10.3969/j.issn.2095-4344.2015.21.010

Abstract

Abstract:

BACKGROUND: Studies on encapsulated whole cell protein antigen of Helicobacter pylori are still at the exploration stage. There is limited literature concerning the preparation process and in vitro release characteristics of chitosan microspheres encapsulated with whole cell protein antigen of Helicobacter pylori.
OBJECTIVE: To explore the preparation process and in vitro release characteristics of chitosan microspheres encapsulating whole cell protein antigen of Helicobacter pylori.
METHODS: Precipitation method was used to prepare chitosan microspheres, and the best preparation process, matching and encapsulation time were screened. Under electron microscope, the morphology and particle size of microspheres were observed. Chitosan microspheres were used to encapsulate Helicobacter pylori whole cell protein antigen, and BCA method was used to determine encapsulation efficiency, encapsulation content and release efficiency in vitro of Helicobacter pylori whole cell protein antigen.
RESULTS AND CONCLUSION: Final concentration of 1% glacial acetic acid, sodium sulfate as crosslinking agent, pH=5.0, with no pulverization when the crosslinking agent was added was the best preparation process for chitosan microspheres. Electron microscopy showed the smooth surface morphology of microspheres with roundness and good dispersion, and the majority of the microspheres were 1-5 μm in diameter. The encapsulation efficiency of Helicobacter pylori whole cell protein antigen microspheres was 80.4%, the encapsulated amount was 16.4%, and total 48-hour release rate was 19.4%. Helicobacter pylori whole cell protein antigen microspheres showed an overall slow release status. Chitosan microspheres show good encapsulation efficiency and amount of Helicobacter pylori whole cell protein antigen, and Helicobacter pylori total bacteria protein antigen microspheres show an overall slow release status.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Biocompatible Materials, Chitosan, Vaccines

CLC Number:

R318

Li Juan, Zhao Qing-xi. Chitosan microspheres loading whole cell protein antigen of Helicobacter pylori: preparation and in vitro release characteristics[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3334-3338.

Figures/Tables 2

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