Expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in Skeletal Class II Malocclusion during growth peak

doi:10.3969/j.issn.2095-4344.2015.20.013

Abstract

Abstract:

BACKGROUND: Preliminary studies of our research group have confirmed that bone morphogenetic protein-4 can stimulate the development of mandible in the growth period, but whether bone morphogenetic protein-4 can interact with bone morphogenetic protein-2 to promote the growth of mandible has not been reported.
OBJECTIVE: To detect the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in skeletal class II malocclusion during growth peak, and to explore the relationship of the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 with mandibular growth.
METHODS: Patients with skeletal class I malocclusion in growth peak served as group I, and those with skeletal class II malocclusion in growth peak characterized as mandibular retrognathia acted as group II. There were 18 cases in each group. Expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in serum was detected by real-time fluorescent quantitative PCR.
RESULTS AND CONCLUSION: The mRNA expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in the group II was significantly lower than that in the group I (P < 0.05). In the group II, there was a significant correlation between the expression of bone morphogenetic protein-2 and bone morphogenetic protein-4. These experimental findings confirm that the reduced expression of bone
morphogenetic protein-2 and bone morphogenetic protein-4 in skeletal class II malocclusion during growth peak has a certain relationship with mandibular deficiency, and moreover, bone morphogenetic protein-2 interacts with bone morphogenetic protein-4 to promote the growth of mandible.

Key words: Malocclusion, Angle Class II, Bone Morphogenetic Proteins, Bone Morphogenetic Protein 4

CLC Number:

R318

Wang Jie, Ma Ce, Wang Yu-rong, Chen De-yu. Expression of bone morphogenetic protein-2 and bone morphogenetic protein-4 in Skeletal Class II Malocclusion during growth peak[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(20): 3183-3187.

Figures/Tables 4

References

[1] 吴增波，王豫蓉.骨形成蛋白-4在骨性Ⅱ类错牙合畸形中的表达[J].中国保健营养，2012，22(6):1232-1233.

[2] 王伟,邱蔚六.骨形成蛋白2诱导骨形成研究进展[J].口腔材料器械杂志,1999,8(2):87-89.

[3] 华松.人骨形成蛋白2基因的克隆及序列测定[J].西北农林科技大学学报,2005,33(2):9-12.

[4] Liua W, Selevera J, Murali D,et al.Threshold-specific requirements for Bmp4 in mandibular development. Developmental Biology.2005;283:282-293.

[5] Ekanayake S, Hall BK.The in vivo and in vitro effects of Bone Morphogenetic Protein-2 on the development of the chick mandible.Int J Dev Biol.1997;41: 67-81.

[6] Tomo S, Ofita M, Toma I.Development of Mandibular Cartilages in the Rat.The Anatomical Record.1997; 249:233-239.

[7] Suzuki T, Bessho K, Fujimura K,et al.Regeneration of defects in the articular cartilage in rabbit temporomandibular joints by bone morphogenetic protein-2. Br J Oral Maxillofac Surg. 2002; 40(3):201-206.

[8] Tsuji K, Bandyopadhyay A, Harfe BD,et al.BMP2 activity, although dispensable for bone formation,is required for the initiation of fracture healing.nature genetics.2006; 12(38): 1424-1429.

[9] de Mara CS, Duarte AS, Sartori-Cintra AR,et al. Coimbra. Chondrogenesis from umbilical cord blood cells stimulated with BMP-2 and BMP-6.Rheumatol Int.2013;33: 121-128.

[10] Sailor LZ, Hewick RM, Morris EA.Recombinant human bone morphogenetic protein-2 maintains the articular chondrocyte phenotype in long-term culture.J Orthop Res. 1996;14(6): 937-945.

[11] 傅民魁.口腔正畸专科教程[M].北京:人民卫生出版社, 2007: 26-58.

[12] 陈扬熙.口腔正畸学-基础、技术与临床[M].人民卫生出版社, 2012: 588-600.

[13] Liu X, Zeng B, Zhang C.Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene.Biotechnol Lett.2011;33:1933-1938.

[14] Bai T, Yang J, Chen B, et al.Genetic analysis of BMP4 gene in Chinese Han female population with premature ovarian insufficiency. Climacteric.2014;17:304-306.

[15] 姜若萍,傅民魁.安氏Ⅱ类1分类错牙合患者亲子间相似性的个体研究[J].中华口腔医学杂志.2001,36(2):143-145.

[16] Peck S, Peck L, Kataja M. Class II Division 2 malocclusion: a heritable pattern of small teeth in well-developed jaws. Angle Orthod. 1998;68(1):9-20.

[17] Chou ST, Tseng YC, Pan CY, et al.Craniofacial Skeletal Dysplasia of Opposite-sex Dizygotic Twins.J Formos Med Assoc. 2011;110(5):342-346.

[18] Cruz RM, Krieger H, Ferreira R, et al.Major Gene and Multifactorial Inheritance of Mandibular Prognathism. Am J Med Genet A. 2008;146A(1):71-77.

[19] Ngo TQ, Scherer MA, Zhou FH, et al.Expression of Bone Morphogenic Proteins and Receptors at the Injured Growth Plate Cartilage in Young Rats. J Histochem Cytochem. 2006; 54(8):945-954.

[20] Shu B, Zhang M, Xie R, et al.BMP2, but not BMP4, is crucial for chondrocyte, proliferation and maturation during endochondral bone development. J Cell Sci. 2011;124(Pt 20): 3428-3440.

[21] Mi M, Jin H, Wang B,et al.Chondrocyte BMP2 signaling plays an essential role in bone fracture healing.Gene.2013;512: 211-218.

[22] De Luca F, Barnes KM, Uyeda JA, et al.Regulation of Growth Plate Chondrogenesis by Bone Morphogenetic Protein-2. Endocrinology.2001;142:430-436.

[23] Bennett JH, Hunt P, Thorogood P..Bone Morphogenetic Protein-2 And -4 Expression During Murine Orofacial Development.Archs oral biol.1995;40(9):847-854.

[24] Bonilla-Claudio M, Wang J, Bai Y,et al.Bmp signaling regulates a dose-dependent transcriptional program to control facial skeletal development. Development. 2012;139(4): 709-719.

[25] MacDonald ME, Hall BK.Hall.Altered Timing of the Extracellular-Matrix-Mediated Epithelial-Mesenchymal Interaction That Initiates Mandibular Skeletogenesis in Three Inbred Strains of Mice: Development, Heterochrony, and Evolutionary Change in Morphology.J Exp Zool.2001;291(3): 258-273.

[26] Rodríguez-Vázquez JF, Mérida-Velasco JR, Mérida-Velasco JA,et al.Development of Meckel’s Cartilage in the Symphyseal Region in Man. Anat Rec. 1997;249:249-254.

[27] 罗应伟,李松,沈丽宁.Meckel’s软骨在下颌骨发育中作用的初步研究[J].昆明医学院学报,2005,1:13-16.

[28] Wang Y, Zheng Y, Chen D, et al.Enhanced BMP signaling prevents degeneration and leads to endochondral ossification of Meckel’s cartilage in mice.Dev Biol.2013;381(2): 301-311.

[29] Tsuji K, Bandyopadhyay A, Harfe BD, et al.BMP2 activity, although dispensable for bone formation,is required for the initiation of fracture healing.nature genetics.2006;12(38): 1424-1429.

[30] Urist MR, Strates BS.Strates.The Classic Bone Morphogenetic Protein.Clin Orthop Relat Res.2009; 467: 3051-3062.

[31] Barnouti ZP, Owtad P, Shen G, et al.The biological mechanisms of PCNA and BMP in TMJ adaptive remodeling. Angle Orthod. 2011;81(1):91-99.

[32] Yang W, Guo D, Harris MA,et al.Bmp2 in osteoblasts of periosteum and trabecular bone links bone formation to vascularization and mesenchymal stem cells.J Cell Sci. 2013 Sep 15;126(Pt 18):4085-4098.

[33] Xie G, Sun J, Zhong G, et al.Hydroxyapatite nanoparticles as a controlled-release carrier of BMP-2:absorption and release kinetics in vitro.Science.2010;21:1875-1880.