Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (11): 1700-1705.doi: 10.3969/j.issn.2095-4344.1106

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Effects of Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor on the expression of osteocalcin and bone morphogenetic protein 2 during early extremity fracture healing in rabbit models of radical fractures

Chen Haitao, An Yuguang   

  1.  (Department of Surgery, People’s Hospital of Yongning District of Nanning City, Nanning 530299, Guangxi Zhuang Autonomous Region, China)
  • Received:2018-11-10 Online:2019-04-18 Published:2019-04-18
  • Contact: An Yuguang, Master, Attending physician, Department of Surgery, People’s Hospital of Yongning District of Nanning City, Nanning 530299, Guangxi Zhuang Autonomous Region, China
  • About author:Chen Haitao, Master, Chief physician, Department of Surgery, People’s Hospital of Yongning District of Nanning City, Nanning 530299, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Scientific Research and Technological Development Project of Nanning City of Guangxi Zhuang Autonomous Region, No. 20153087 (to CHT)

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Abstract:

ACKGROUND: Huoxue Jiegu Decoction has been shown to be effective for extremity fractures, but the antalgic efficacy of Chinese compounds during early fracture healing is poorer than that of western medicines.
OBJECTIVE: To study the effects of Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor on the levels of osteocalcin and bone morphogenetic protein during early extremity fracture healing.
METHODS: Sixty New Zealand rabbits (3-5 months old, (2.5±0.5) kg) provided by Laboratory Animal Center of Guangxi Medical University were included. Rabbit models of radical fracture were established, and then randomized into cyclooxygenase-2 inhibitor group, blank control group, and Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor group. Serum osteocalcin level was determined by ELISA at 1, 2, 3, 4, and 6 weeks postoperatively. The expression levels of bone morphogenetic protein 2 in the outer and inner periosteum were detected by immunohistochemical staining at different time points of fracture healing, and the average optical density value of positive cells was analyzed.
RESULTS AND CONCLUSION: The levels of osteocalcin at 2, 3, 4 and 6 weeks after fracture in the Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor group were significantly higher than those in the other two groups (P < 0.05). The average optical density value of bone morphogenetic protein 2 in the outer and inner periosteum and bone marrow cavity at 2, 3, and 4 weeks in the Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor group were significantly higher than those in the other two groups (P < 0.05). These results indicate that Huoxue Jiegu Decoction combined with cyclooxygenase-2 inhibitor can promote the early fracture healing to some extent, and increase the expression of osteocalcin and bone morphogenetic protein 2. The mechanism may be related to increased osteocalcin and bone morphogenetic protein 2 expression.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Radical Fractures, Cyclooxygenase 2, Osteocalcin, Bone Morphogenetic Proteins, Tissue Engineering

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