Antimicrobial and sealant properties of nanohydroxyapatite as endodontic sealer

doi:10.3969/j.issn.2095-4344.2014.21.013

Abstract

Abstract:

BACKGROUND: Depending on different pH values, nanohydroxyapatite materials have different purities, whether root canal sealers formed by which exert effects on antimicrobial resistance, root canal closure and apical histocompatibility has no reports.
OBJECTIVE: To compare the antimicrobial and sealant properties of nanohydroxyapatite materials with different pH, nanohydroxyapatite-composite and traditional root canal sealants.
METHODS: We detected the antimicrobial action of nanohydroxyapatite with pH 8, 9, 10, Vitapex and AH-Plus root canal sealants with and without addition of ornidazole against three microbial strains namely Enterococcus faecalis, Candida albicans and Staphylococcus aureus using agar diffusion method. And we also analyzed the endodontic microleakage of six root canal sealants by determining the apical reservoir glucose concentration using Glucose Oxidase Method.
RESULTS AND CONCLUSION: Pure nanohydroxyapatite with different pH did not show antimicrobial properties. Addition of ornidazole to nanohydroxyapatite showed greater inhibitory action against Enterococcus faecalis, lesser in Staphylococcus aureus, followed by Candida albicans. Vitapex root canal sealer had inhibitory effects only against Staphylococcus aureus. AH-Plus, itself, had antimicrobial activity against all the three strains, but the antimicrobial activity decreased after addition of ornidazole. Nanohydroxyapatite, as a root canal sealant, was superior to zinc oxide eugenol and Vitapex, but inferior to AH-Plus. Addition of ornidazole to nanohydroxyapatite for a short period showed no impact on sealant properties of the material.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, nanoparticles, durapatite, ointments

CLC Number:

R318

Wang Jian-ping, Geogi George. Antimicrobial and sealant properties of nanohydroxyapatite as endodontic sealer[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3350-3354.

Figures/Tables 3

References

[1]Poursamara SA, Rabieea M, Samadikuchaksaraeib A, et al. Influence of the value of the pH on the preparation of nano hydroxyapatite-poly vinyl alcohol composites. J Ceram Process Res. 2009;10(5):679-682.
[2]Asgary S, Kamrani FA. Antibacterial effects of five different root canal sealing materia. J Oral Sci. 2008;50(4):469-474.
[3]Widowati W, Naing L, Ema M, et al. Sealing ability of hydroxyapatite as a root canal sealer: in vitro study. MJMS. 2007;40(3):101-105.
[4]Cobankara FK, Altinöz HC, Ergani O, et al. In vitro antibacterial activities of rootcanal sealers by using two different methods. J Endod. 2004;30:57-60.
[5]Sipert CR, Hussne RP, Nishiyama CK, et al. In vitro antimicrobial activity of Fill Canal, Sealapex, Mineral Trioxide Aggregate, Portland cement and EndoRez. Int Endod J. 2005;38:539-543.
[6]Siqueira JF Jr, Favieri A, Gahyva SM, et al. Antimicrobial activity and ?ow rate of newer and established root canal sealers. J Endod. 2000;26:274-277.
[7]Heling I, Chandler NP. The antimicrobial effect within dentinal tubules of four root canal sealers. J Endod. 1996; 22: 257-259.
[8]Cobankara FK, Altinoz HC, Ergani O, et al. In vitro antibacterial activities of root-canal sealers by using two different methods. J Endod. 2004;30:57-60.
[9]Zhang H, Shen Y, Ruse ND, et al. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod. 2009;35(7):1051-1055.
[10]Ma XD. The observation of short term effect of spherical 20 nm hydroxyapatite root canal filling materials. Kouqiang Yixue. 2010;30(2):121-122.
[11]Ma XD, Wang JP. Basic research of nano hydroxyapatite (nHA) root canal filling materials. Xinan Junyi. 2009;11(3):485-487.
[12]Du C, Cui Fz, Zhang W, et al. Formation of calcium phosphate/collagen composites through mineralization of collagen matrix. J Biomed Mater Res. 2000;50(4):518-527.
[13]Du C, Cui Fz, Feng QL, et al. Tissue response to nano-hydroxyapatite/collagen composite implants in marrow cavity. J Biomed Mater Res. 1998;42:540-548.
[14]Wesselink P. Root filling techniques. In: Bergenholtz. G, Bindslev PH, Reit Claes, eds. Text Book of Endodontology. Oxford: Blackwell Publishing Company, 2003:286-290.
[15]Timpawat S, Amornchat C, Trisuwan WR. Bacterial coronal leakage after obturation with three root canal sealers. J endod. 2001;27(1):36-39.
[16]Oliver CM, Abbot PV. An in vitro study of apical and coronal microleakage of laterally condensed gutta-percha with Ketac-endo and AH-26. Aust Dent J. 1998;43:262-268.
[17]Cobankara FK, Adanir N, Belli S, et al. A quantitative evaluation of apical leakage of four root-canal sealers. Int endod J. 2002;35(12):979-984.