Preosteoblastic differentiation of MC3T3 E1 cells on the new-type self-assembling peptide hydrogel NBD/RADA16

doi:10.3969/j.issn.2095-4344.2014.21.009

Abstract

Abstract:

BACKGROUND: RADA16 is a mature amphiphilic self-assembling peptide, which can be assembled into nanofibers, and promote MC3T3 E1 cell attachment, spreading and proliferation.
OBJECTIVE: To observe the effects of the new-type self-assembling peptide hydrogel NBD/RADA16 on osteogenic differentiation of mouse preosteoblasts MC3T3 E1.
METHODS: The MC3T3 E1 cells were inoculated in NBD/RADA16 self-assembling peptide hydrogel and RADA16 hydrogel for osteogenic induction. Cells undergoing simple osteogenic induction served as controls. After culture for 1, 3, 6 days, the activity of alkaline phosphatase was detected. After 7 days, western blot assay was used to determine the expression of bone morphogenetic protein-2. After 21 days, alizarin red staining was used to observe calcified nodules.
RESULTS AND CONCLUSION: MC3T3 E1 cells grew well on the NBD/RADA16 peptide hydrogel, which were superior to those on the RADA16 hydrogel. The activity of alkaline phoshpatase was higher in the NBD/RADA16 group than the RADA16 and control groups (P < 0.01). Compared with the RADA16 hydrogel, mineralized matrix deposition and expression of bone morphogenetic protein-2 were higher on the NBD/RADA16 peptide hydrogel (P < 0.01). These findings indicate that the NBD/RADA16 peptide hydrogel is superior to the RADA16 hydrogel for promoting the osteogenic differentiation of MC3T3 E1 cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: hydrogel, peptides, osteoblasts

CLC Number:

R318

Zhao Gang, Li Jing-ling, Wang Dan, Yu Jing, Zhao Yan-yu, Man Da-peng. Preosteoblastic differentiation of MC3T3 E1 cells on the new-type self-assembling peptide hydrogel NBD/RADA16[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3329-3333.

Figures/Tables 3

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