Modification of superhydrophobic surface of TiAl6Vi4 and its bacteriostatic action in vitro

doi:10.3969/j.issn.2095-4344.2014.21.008

Abstract

Abstract:

BACKGROUND: Studies have shown that the surface hydrophilicity and hydrophobicity (i.e., surface wettability) are important factors affecting bacterial adhesion.
OBJECTIVE: To observe the bacteriostatic action on Staphylococcus aureus after changing wettability on TiAl₆Vi₄ plates by surface modification.
METHODS: TiAl₆Vi₄ plates were buffered and polished, then divided into three groups, ten plates in each group. T1 group was prepared for TiO₂ nanometer film on TiAl₆Vi₄ surface by electrochemically anodic oxidation method, and self-assembled PETS. T2 group was only prepared for TiO₂ nanometer film. T3 group was only self-assembled PETS. All the groups were observed by contact angle measurements and Staphylococcus aureus adhesion on different surfaces was evaluated immersion in Staphylococcus aureus solution for 2 hours in vitro.
RESULTS AND CONCLUSION: Staphylococcus aureus on the plates with hydrophilic surfaces (T1 group) aggregated reciprocally and overlapped together in the shape of grape cluster while those on the hydrophobic surfaces (T2 group) displayed a tendency of aggregation, however resulting in monolayer but not in grape-cluster like shape. On the contrary, bacteria on the plates with superhydrophobic surfaces (T3 group) distributed dispersedly with no bacteria-clusters or strings. Furthermore, along with the decline of hydrophilia, bacteria counts decreased gradually, exhibiting an increasing trend of separation among bacteria as well. Absorbance value of T3 group remained significantly higher than that of T1 and T2 groups (P < 0.05). It is indicated that titanium superhydrophobic surfaces are able to reduce Staphylococcus aureus adhesion and useful for prevention implant related infection of orthopedics.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, titanium, Staphylococcus aureus, hydrophobic and hydrophilic interactions

CLC Number:

R318

Zhang Wei, Zhang Li-cheng, Zhang Li-hai, Wei Jun-qiang, Tang Pei-fu . Modification of superhydrophobic surface of TiAl₆Vi₄and its bacteriostatic action in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3322-3328.

Figures/Tables 7

References

[1]Williams D.The golden anniversary of titanium biomaterials.Med Device Technol. 2001;12(7):8-11.
[2]Ferguson SJ,Langhoff JD,Voelter K,et al.Biomechanical comparison of different surface modifications for dental implants.Int J Oral Maxillofac Implants. 2008;23(6): 1037-1046.
[3]Vadillo-Rodriguez V,Bruque JM,Gallardo-Moreno AM,et al.Surface-dependent mechanical stability of adsorbed human plasma fibronectin on Ti6Al4V: domain unfolding and stepwise unraveling of single compact molecules. Langmuir : the ACS journal of surfaces and colloids.2013;29(27): 8554-8560.
[4]Zhao Y,Wong SM,Wong HM,et al.Effects of carbon and nitrogen plasma immersion ion implantation on in vitro and in vivo biocompatibility of titanium alloy. ACS Appl Mater Interfaces. 2013;5(4):1510-1516.
[5]Fischer U,Hempel U,Becker D,et al.Transforming growth factor beta1 immobilized adsorptively on Ti6Al4V and collagen type I coated Ti6Al4V maintains its biological activity. Biomaterials.2003;24(15):2631-2641.
[6]Leventhal GS.Titanium, a metal for surgery. J Bone Joint Surg Am.1951;33-a(2):473-474.
[7]De Nardo L,Raffaini G,Ebramzadeh E,et al.Titanium oxide modeling and design for innovative biomedical surfaces: a concise review.Int J Artif Organs.2012;35(9):629-641.
[8]Murr LE,Quinones SA,Gaytan SM,et al.Microstructure and mechanical behavior of Ti-6Al-4V produced by rapid-layer manufacturing, for biomedical applications. J Mech Behav Biomed Mater.2009;2(1):20-32.
[9]Minagar S,Wang J,Berndt CC,et al. Cell response of anodized nanotubes on titanium and titanium alloys.J Biomed Mater Res A.2013;101(9):2726-2739.
[10]Abdel-Hady Gepreel M,Niinomi M.Biocompatibility of Ti-alloys for long-term implantation.J Mech Behav Biomed Mater.2013; 20:407-415.
[11]Vanderleyden E,Mullens S,Luyten J,et al.Implantable (bio)polymer coated titanium scaffolds: a review.Curr Pharm Des.2012;18(18):2576-2590.
[12]Saharudin KA,Sreekantan S,Abd Aziz SN,et al.Surface modification and bioactivity of anodic Ti6Al4V alloy.J Nanosci Nanotechnol.2013;13(3):1696-1705.
[13]Niinomi M,Nakai M,Hieda J.Development of new metallic alloys for biomedical applications. Acta Biomaterialia. 2012; 8(11):3888-3903.
[14]Hanawa T.A comprehensive review of techniques for biofunctionalization of titanium.J Periodontal Implant Sci. 2011;41(6):263-272.
[15]Nakai M,Niinomi M,Akahori T,et al.Development of biomedical porous titanium filled with medical polymer by in-situ polymerization of monomer solution infiltrated into pores.J Mech Behav Biomed Mater.2010;3(1):41-50.
[16]Yilmazer H,Niinomi M,Nakai M,et al.Heterogeneous structure and mechanical hardness of biomedical beta-type Ti-29Nb-13Ta-4.6Zr subjected to high-pressure torsion.J Mech Behav Biomed Mater.2012;10:235-245.
[17]Niinomi M,Nakai M,Akahori T.Frictional wear characteristics of biomedical Ti-29Nb-13Ta-4.6Zr alloy with various microstructures in air and simulated body fluid. Biomed Mater. 2007;2(3):S167-174.
[18]Nakai M,Niinomi M,Ishii D.Mechanical and biodegradable properties of porous titanium filled with poly-L-lactic acid by modified in situ polymerization technique. J Mech Behav Biomed Mater.2011;4(7):1206-1218.
[19]Tanaka Y,Matsuo Y,Komiya T,et al.Characterization of the spatial immobilization manner of poly(ethylene glycol) to a titanium surface with immersion and electrodeposition and its effects on platelet adhesion.J Biomed Mater Res A.2010; 92(1):350-358.
[20]Nowicka J,Bartoszewicz M,Gosciniak G.[Effect of selected properties of Staphylococcus epidermidis to biofilm formation on orthopedic implants]. Med Dosw Mikrobiol. 2012;64(3): 189-196.
[21]Ji J,Zhang W. Bacterial behaviors on polymer surfaces with organic and inorganic antimicrobial compounds.J Biomed Mater Res A.2009;88(2):448-453.
[22]Raulio M,Jarn M,Ahola J,et al. Microbe repelling coated stainless steel analysed by field emission scanning electron microscopy and physicochemical methods.J Ind Microbiol Biotechnol.2008;35(7):751-760.
[23]Okada A,Nikaido T,Ikeda M,et al.Inhibition of biofilm formation using newly developed coating materials with self-cleaning properties. Dent Mater J. 2008;27(4):565-572.
[24]Huan Z,Fratila-Apachitei LE,Apachitei I,et al. Porous TiO? surface formed on nickel-titanium alloy by plasma electrolytic oxidation: a prospective polymer-free reservoir for drug eluting stent applications.J Biomed Mater Res B Appl Biomater. 2013;101(5):700-708.
[25]Antoci V Jr,Adams CS,Hickok NJ,et al.Vancomycin bound to Ti rods reduces periprosthetic infection: preliminary study.Clin Orthop Relat Res.2007;461:88-95.
[26]Neogi DS,Yadav CS,Khan SA,et al.In vivo efficacy of antimicrobial-coated devices. J Bone Joint Surg.2008; 90(8): 1785; author reply 1785-1786.
[27]An YH,Stuart GW,McDowell SJ,et al. Prevention of bacterial adherence to implant surfaces with a crosslinked albumin coating in vitro.J Orthop Res.1996;14(5):846-849.
[28]Arciola CR,Radin L,Alvergna P,et al.Heparin surface treatment of poly(methylmethacrylate) alters adhesion of a Staphylococcus aureus strain: utility of bacterial fatty acid analysis.Biomaterials.1993;14(15):1161-1164.
[29]Chandy T,Sharma CP.Chitosan--as a biomaterial. Biomater Artif Cells Artif Organs. 1990;18(1):1-24.
[30]Chandy T,Sharma CP.Chitosan matrix for oral sustained delivery of ampicillin. Biomaterials.1993;14(12):939-944.
[31]Wassall MA,Santin M,Isalberti C,et al.Adhesion of bacteria to stainless steel and silver-coated orthopedic external fixation pins.J Biomed Mater Res.1997;36(3):325-330.
[32]Dell'Acqua G,Giacometti A,Cirioni O,et al.Suppression of drug-resistant Staphylococcal Infections by the quorum-sensing inhibitor RNAIII-inhibiting peptide. J Infect Dis.2004;190(2):318-320.
[33]Lerebour G,Cupferman S,Bellon-Fontaine MN.Adhesion of Staphylococcus aureus and Staphylococcus epidermidis to the Episkin reconstructed epidermis model and to an inert 304 stainless steel substrate.J Appl Microbiol.2004;97(1):7-16.
[34]Lafuma A,Quéré D.Superhydrophobic states. Nat Mater.2003; 2(7):457-460.
[35]Lewis K. Persister cells, dormancy and infectious disease. Nature reviews. Microbiology. Jan 2007;5(1):48-56.
[36]Bernhardt R,Scharnweber D,Muller B,et al.Comparison of microfocus- and synchrotron X-ray tomography for the analysis of osteointegration around Ti6Al4V implants.Eur Cell Mater.2004;7:42-51; discussion 51.
[37]Bernhardt R,Kuhlisch E,Schulz MC,et al.Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices.Eur Cell Mater.2012;23:237-247;discussion 247-248.
[38]Shalabi MM,Wolke JG,Cuijpers VM,et al.Evaluation of bone response to titanium-coated polymethyl methacrylate resin (PMMA) implants by X-ray tomography.J Mater Sci Mater Med.2007;18(10):2033-2039.
[39]Kiba H,Hayakawa T,Oba S,et al.Potential application of high-resolution microfocus X-ray techniques for observation of bone structure and bone-implant interface.Int J Oral Maxillofac Implants.2003;18(2):279-285.
[40]Cancedda R,Cedola A,Giuliani A,et al.Bulk and interface investigations of scaffolds and tissue-engineered bones by X-ray microtomography and X-ray microdiffraction. Biomaterials. 2007;28(15):2505-2524.

Modification of superhydrophobic surface of TiAl₆Vi₄and its bacteriostatic action in vitro

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Li Xingping, Xiao Dongqin, Zhao Qiao, Chen Shuo, Bai Yiguang, Liu Kang, Feng Gang, Duan Ke. Preparation and properties of copper-loaded antibacterial functional film on titanium surface [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 553-557.
[2]	Shi Xiaoxiu, Mao Shilong, Liu Yang, Ma Xingshuang, Luo Yanfeng. Comparison of tantalum and titanium (alloy) as orthopedic materials: physical and chemical indexes, antibacterial and osteogenic ability [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 593-599.
[3]	Zhang Xianjun, Zhao Xijiang. In vivo osteogenic properties of silicon-incorporated titanium dioxide nanotubes on titanium screw surface [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2461-2465.
[4]	Liu Zige, Liu Xinrui, Li Yan, Song Guorui, Zhang Chen, Chen Desheng. In vitro experiment of tetrandrine on the model of osteolysis induced by wear particles around the prosthesis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(15): 2358-2363.
[5]	Wu Shengxiang, Liu Yuan, Lu Shuai. Mini-locking titanium plate system fixation in the treatment of carpal scaphoid fracture [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(12): 1874-1878.
[6]	Zhang Lixing, Tian Ang, Li Xi, Bai Xizhuang. Drug-release characteristic and biological toxicity of TiO₂ nanotube/hydroxyapatite loaded vancomycin coating [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(10): 1500-1506.
[7]	Gu Yuanping, Lu Chenghui . Cleaning efficacy of two kinds of mechanical nickel-titanium instruments in preparation of severely curved root canals: a scanning electron microscopic study [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(10): 1566-1570.
[8]	Ma Jinchao, Liu Tiansheng, Liu Aipeng, Wang Hao, Wang Qi, Liang Yongjian, Wang Lin, Di Haiwei. Photodynamic antimicrobial chemotherapy for repairing a rabbit model of osteomyelitis [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(8): 1254-1259.
[9]	Liu Chundong, Shen Xiaoqing, Zhang Yanli, Zhang Xiaogen, Wu Buling. Effects of strontium-modified titanium surfaces on adhesion, migration and proliferation of bone marrow mesenchymal stem cells and expression of bone formation-related genes [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(7): 1009-1015.
[10]	Yang Xu, Zhao Xiaofeng, Qi Detai, Wang Xiaonan, Jin Yuanzhang, Zhou Runtian, Zhao Bin. Changes in cervical sagittal balance after three-dimensional printing ACT titanium cage in anterior cervical discectomy with fusion [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(36): 5741-5748.
[11]	Zhang Hui, Xu Nanwei, Nong Luming, Tang Xueming, Zhou Xindie, Jiang Wei. Factors influencing the prognosis of central cord syndrome treated with drug therapy and titanium plate fixation [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(3): 348-353.
[12]	Yang Xiao, Mei Wei, Zhang Wei. Absorbable rod or titanium alloy screws for Mason type II radial head fractures [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(27): 4328-4332.
[13]	Zhang Xuan, Li Yunpeng, Zhang Xuejian, Yin Chuanrong, Deng Yue. Guided bone regeneration using preformed titanium mesh combined with bioabsorbable membranes in aesthetic area [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(26): 4112-4117.
[14]	Zhang Lan, Wang Xiang, Liu Jun, Zhang Chunqiu, Ye Jinduo, Liu Lu. Tensile properties of three-dimensional printed porous titanium alloy trabecular bone [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(22): 3498-3503.
[15]	Wang Yanling, Shao Zhe, He Wei. Micro-arc oxidation and osteoblast proliferation and osteogenic differentiation ability on titanium surface [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(22): 3486-3490.