Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (22): 3486-3490.doi: 10.3969/j.issn.2095-4344.2290

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Micro-arc oxidation and osteoblast proliferation and osteogenic differentiation ability on titanium surface

Wang Yanling1, Shao Zhe2, He Wei3   

  1. 1Department of Stomatology, Henan Province Hospital of TCM (Second Affiliated Hospital of Henan University of Chinese Medicine), Zhengzhou 450002, Henan Province, China; 2Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei Province, China; 3Department of Stomatology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • Received:2019-09-16 Revised:2019-09-18 Accepted:2019-11-25 Online:2020-08-08 Published:2020-04-26
  • Contact: He Wei, Chief physician, Department of Stomatology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • About author:Wang Yanling, Master, Attending physician, Department of Stomatology, Henan Province Hospital of TCM (Second Affiliated Hospital of Henan University of Chinese Medicine), Zhengzhou 450002, Henan Province, China
  • Supported by:
    the Science and Technology Development Program of Science and Technology Department of Henan Province, Nos. 142300410086  and 172102310613

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Abstract:

BACKGROUND: Electrochemical method based nanoscale hydroxyapatite coating has slow degradation speed (8-12 weeks). Micro-arc oxidation based method could form homogeneous coating on complex surfaces, and this coating promotes cell adhesion and ingrowth in bone tissues.

OBJECTIVE: To explore the effect of micro-arc oxidation hydroxyapatite coating titanium alloy on osteoblast proliferation and osteogenic differentiation ability.

METHODS: Hydroxyapatite coating titanium alloy materials were prepared by electrochemical method and micro-arc oxidation method. The contact angles of the two materials were detected. Osteoblasts (hFOB1.19) were incubated on the hydroxyapatite coating titanium alloy materials for 48 hours. The morphological changes of osteoblasts on the materials were observed under scanning electron microscope. The cell proliferation was detected by MTT method at 1, 12, 24, 48 and 72 hours. The cell count and alkaline phosphatase activity were detected at 1, 3 and 5 days of culture. At 5 days, the expression levels of bone morphogenetic proteins 2 and 4 were detected by western blot assay.

RESULTS AND CONCLUSION: (1) The contact angle in the micro-arc oxidation group was smaller than that in the electrochemical group [(66.5±2.2)°, (52.8±2.1)°, P=0.001 5)]. (2) Scanning electron microscope revealed that the osteoblasts in the electrochemical group had an irregular and shrunken shape, and adhered loosely to the material surface. The osteoblasts in the micro-arc oxidation had a fully outstretched and flat shape, and adhered tightly to the material surface. (3) From 12 to 72 hours, the cell proliferation in the micro-arc oxidation group was faster than that in the electrochemical group (P < 0.05). At 3 and 5 days after culture, the cell proliferation in the micro-arc oxidation group was faster than that in the electrochemical group (P < 0.05). (4) At 1, 3 and 5 days, the alkaline phosphatase activity of osteoblasts in the micro-arc oxidation group was higher than that in the electrochemical group (P < 0.05). (5) The expression levels of bone morphogenetic proteins 2 and 4 were significantly up-regulated in the micro-arc oxidation group compared with the electrochemical group (P < 0.05). (6) These results indicate that micro-arc oxidation hydroxyapatite coated titanium alloy increases osteoblast proliferation and osteogenic differentiation ability.

Key words:

"> implants, osteoblasts, micro-arc oxidation, titanium, electrochemistry, osteogenic differentiation, alkaline phosphatase, bone morphogenetic protein

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