Effect of cyclic stretch on the proliferation of C2C12 myoblasts

doi:10.3969/j.issn.2095-4344.2014.20.023

Abstract

Abstract:

BACKGROUND: Mechanical stimulation triggers a muscle tissue remodeling, during which, myoblast process is a multi-stage development process. Many signals in extracellular matrix are involved in myoblast process, and mechanical signals are considered as important external factors for muscle formation and regeneration. Numerous studies concerning cyclic tensile stress effects on myoblast proliferation and apoptosis have been reported, but the specific mechanism underlying the mechanical stimulation in muscle formation is still unclear.
OBJECTIVE: To study the effects of cyclic stretch on proliferation of C2C12 myoblasts and its mechanism.
METHODS: C2C12 myoblasts were cultured in vitro under 10% cyclic stretch for 6, 12 and 24 hours.
RESULTS AND CONCLUSION: The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that with the time of cyclic stretch, the proliferation of C2C12 myoblasts and the rate of S-phase fraction were gradually increased and peaked at 12 hours (P < 0.05), and then decreased. Western blot assay showed that the expression of nuclear transcription factor κB protein was decreased with time, which was inversely correlated to the proliferation of C2C12 myoblasts, but increased at 24 hours under the cyclic stretch. These findings indicate that the cyclic stretch can induce C2C12 myoblasts proliferation and alter the cell cycle. Nuclear transcription factor κB may be involved in this process.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: myoblasts, periodicity, elasticity, cell count, cells

CLC Number:

R318

Wang Hong-ling, Liu Dong-xu, Yuan Xiao. Effect of cyclic stretch on the proliferation of C2C12 myoblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3242-3245.

Figures/Tables 1

References

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