Effect of basic fibroblast growth factor on human periodontal ligament cells cultured in vitro

doi:10.3969/j.issn.2095-4344.2014.20.013

Abstract

Abstract:

BACKGROUND: Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells.
OBJECTIVE: To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro.
METHODS: Human periodontal ligament cells at passage 5 were inoculated into the 96-well plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured in α-MEM containing 15% fetal bovine serum and 0, 1, 10, 100 μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cell proliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days.
RESULTS AND CONCLUSION: There were significant differences in the proliferation of human periodontal ligament cells among the four groups (F=6.586, P=0.024). As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradually increased and reached the peak in 100 μg/L basic fibroblast growth factor group (P < 0.05). The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group (P=0.000), the higher the concentration was, the lower activity was (P < 0.05). Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.

Key words: periodontal ligament, cells, fibroblast growth factor 2, cell proliferation

CLC Number:

R318

Lin Yang-dong, Zhao Bo, Yin Kai. Effect of basic fibroblast growth factor on human periodontal ligament cells cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3184-3189.

Figures/Tables 1

References

[1] 王新,戴泓,邹建明,等.双黄补和转化生长因子β1对牙周膜细胞的增殖分化的调控作用的比较[J].中国美容医学, 2009,18(11): 1630-1632.
[2] 刘娟,赵宏宇,轩东英,等.人牙周膜细胞群多向分化潜能的实验研究[J].华西口腔医学杂志,2010,28(2):185-189.
[3] Liu L, Ling J, Wei X,et al.Stem cell regulatory gene expression in human adult dental pulp and periodontal ligament cells undergoing odontogenic/osteogenic differentiation.J Endod. 2009;35(10):1368-1376.
[4] 姚春宇,孙晓菊,谢洪,等.OGP和IGF-1对人牙周膜细胞增殖和总蛋白含量的影响[J].中国美容整形外科杂志, 2011,22(4): 243-246.
[5] 赵寰,丁一,杨禾,等.多肽生长因子对牙周膜细胞的影响[J].中国修复重建外科杂志,2007,21(2):171-174.
[6] Liu J, Zha HY, Xuan DY,et al.Differentiation characteristics of human periodontal ligament cell population in vitro.Hua Xi Kou Qiang Yi Xue Za Zhi. 2010;28(2):185-189.
[7] Flores MG, Yashiro R, Washio K,et al.Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats.J Clin Periodontol. 2008;35(12):1066-1072.
[8] Tan Z, Gong P, Zhao Q.Influence of basic fibroblast growth factors on the migration, proiferation of osteoblasts and periodontal ligament fibroblasts.Hua Xi Kou Qiang Yi Xue Za Zhi. 2005;23(3):201-203.
[9] 陈莹,林崇韬,高颖.碱性成纤维细胞生长因子对牙周膜细胞表皮生长因子受体基因表达的影响[J].华西口腔医学杂志, 2009, 27(3):340-343.
[10] 许彦枝,邹慧儒,王小玲,等.中药双黄补对人牙周膜细胞总蛋白含量和超微结构的影响[J].实用口腔医学杂志, 2005,21(3): 374-377.
[11] 李京平,凌均棨.肿瘤坏死因子和脂多糖影响牙周膜细胞表达骨保护因子的研究[J].中华口腔医学杂志,2003,38(4):288-291.
[12] 张凤秋,吴织芬,万玲,等.牙周优势菌内毒素对人牙周膜细胞增殖和碱性磷酸酶活性的影响[J].牙体牙髓牙周病学杂志, 2003, 13(1):27-29.
[13] 杨在章,武云霞,张华屏,等.碱性成纤维细胞生长因子对人牙周膜细胞合成层黏连蛋白的影响[J].山西医药杂志, 2009,38(2): 129-130.
[14] 周洁,李静,宋锦璘,等.低强度脉冲超声对人牙周膜细胞增殖及碱性磷酸酶活性的影响[J].中国超声医学杂志,2013,29(3): 260-263.
[15] 丁茜,张凤秋.淫羊藿苷对人牙周膜细胞表达核因子κB受体激活蛋白配体和护骨因子的影响[J].中华口腔医学杂志, 2013,48(2): 68-71.
[16] 黄伟琨,管晓燕,刘建国,等.银杏叶提取物对脂多糖作用下人牙周膜细胞增殖及分泌细胞因子的影响[J].中华口腔医学杂志, 2013, 48(Z1):77-82.
[17] 赵艳红,李洪发,杨强,等.人重组骨形成蛋白2作用下人牙周膜细胞Osterix的表达变化[J].中华医学杂志,2013,93(14): 1104-1108.
[18] 姜瑛,王祥,许华,等.淫羊藿苷对内毒素抑制牙周膜细胞ALP表达的影响[J].临床口腔医学杂志,2013,29(3):137-140.
[19] Haley EM, Kim Y.The role of basic fibroblast growth factor in glioblastoma multiforme and glioblastoma stem cells and in their in vitro culture.Cancer Lett. 2014;346(1):1-5.
[20] 唐晓琳,刘静波,包穆蓉,等.17-β雌二醇对牙龈卟啉单胞菌作用下人牙周膜细胞表达白细胞介素6、8的影响[J].中华口腔医学杂志,2012,47(6):329-334.
[21] 赵利芬,夏斌,段开文.壳聚糖/葡甘聚糖混合膜对人牙周膜细胞作用观察[J].临床口腔医学杂志,2012,28(7):409-412.
[22] 王欣,房明,刘敏杰,等.颌骨骨髓基质细胞与牙周膜细胞的生物学特性比较[J].牙体牙髓牙周病学杂志,2012,22(2):66-70.
[23] Kinoshita T, Ishikawa Y, Arita M,et al.Antifibrotic response of cardiac fibroblasts in hypertensive hearts through enhanced TIMP-1 expression by basic fibroblast growth factor. Cardiovasc Pathol. 2014;23(2):92-100.
[24] 段立立,许丽华,王洁,等.内毒素对人牙周膜细胞Toll样受体2和Toll样受体4表达的影响[J].牙体牙髓牙周病学杂志, 2012, 22(2):71-73,70.
[25] 房明,汪涛,王欣,等.糖基化终产物对人牙周膜细胞增殖和NF-κB mRNA表达的影响[J].牙体牙髓牙周病学杂志, 2012,22(4): 200-202,226.
[26] 冯琴,张凤秋,孙正,等.牙龈卟啉单胞菌牙龈蛋白酶K对人牙周膜细胞增殖的影响[J].北京口腔医学,2012,20(1):4-6.
[27] Wu J, Mao Z, Han L,et al.A density gradient of basic fibroblast growth factor guides directional migration of vascular smooth muscle cells.Colloids Surf B Biointerfaces. 2014;117C: 290-295.
[28] 崔晓峰,李红.碱性成纤维细胞生长因子对噪声暴露后豚鼠听力损伤的保护作用[J].中国组织工程研究与临床康复,2002,6(6): 810-811.
[29] 孙晓艳,刘惠玲,付小兵.碱性成纤维细胞生长因子对表皮细胞中端粒酶反转录酶表达的影响[J].解放军医学杂志,2011,36(6): 625-628.
[30] 高松哲,陈剑,王彦平,等.碱性成纤维细胞生长因子对体外培养人羊膜间充质干细胞增殖的影响[J].热带医学杂志,2011,11(5): 543-545.
[31] Tanabe K, Matsushima-Nishiwaki R, Iida M,et al.Involvement of phosphatidylinositol 3-kinase/Akt on basic fibroblast growth factor-induced glial cell line-derived neurotrophic factor release from rat glioma cells.Brain Res. 2012;1463:21-29.
[32] 闫嘉晴,林崇韬,申玉芹,等.碱性成纤维细胞生长因子对人牙周膜细胞多配体蛋白聚糖-4基因表达的影响[J].华西口腔医学杂志, 2011,29(6):588-591.
[33] 杨月,赵敏.碱性成纤维细胞生长因子联合透明质酸钠对角膜保存液中内皮细胞的作用[J].中国组织工程研究与临床康复, 2011, 15(2):281-285.
[34] 贾祎佳,刘强,卢向东,等.转碱性成纤维细胞生长因子基因组织工程骨修复兔骨缺损[J].中国组织工程研究与临床康复, 2011, 15(8): 1365-1368.
[35] 王红,高蕾,王静,等.碱性成纤维细胞生长因子对紫外线诱导豚鼠巩膜细胞凋亡的影响及其与凋亡抑制基因bcl-2的相关性研究[J].环境与健康杂志,2011,28(12):1065-1068.
[36] 付海瑞,李平,庞利凯,等.体外冲击波对MG63细胞中碱性成纤维细胞生长因子表达的影响[J].中国药物与临床,2010,10(3): 301-302.
[37] Yan C, Wang L, Pan ZQ.The effect of basic fibroblast growth factor and epidermal growth factor on corneal endothelial cells preserved in mid-term preserving medium.Zhonghua Yan Ke Za Zhi. 2006;42(2):166-170.
[38] 王志勇,陈增海,于胜吉.碱性成纤维细胞生长因子对体外培养的兔骨髓基质细胞生长特性的影响[J].山东大学学报:医学版, 2006,44(11):1090-1094.
[39] 刘宁,刘鲁川,郝立辉,等.复方奥硝唑-甲磺酸培氟沙星缓释制剂对人牙周膜细胞凋亡及超微结构的影响[J].现代生物医学进展, 2011,11(20):3816-3819.
[40] Wu J, Mao Z, Han L,et al.A density gradient of basic fibroblast growth factor guides directional migration of vascular smooth muscle cells.Colloids Surf B Biointerfaces. 2014;117C: 290-295.
[41] 王磊,曹军,骞爱荣,等.模拟失重对人牙周膜成纤维细胞生物学性能的影响[J].航天医学与医学工程,2011,24(2):96-99.
[42] 尤熹文,储雯,雷雅燕,等.蜂胶对人牙周膜成纤维细胞作用的实验研究[J].实用口腔医学杂志,2011,27(3):385-389.
[43] 李昂,赵俊杰,刘瑾,等.联合应用柚皮苷及黄芩苷对人牙周膜细胞生物学活性的影响[J].实用口腔医学杂志,2011,27(5):619-623.
[44] 周丽华,刘亚君,谈珺,等.三种根面洁治方法对人牙周膜细胞再附着和增殖的影响[J].临床口腔医学杂志,2011,27(5):262-265.
[45] Zhu H, Yang A, Du J,et al.Basic fibroblast growth factor is a key factor that induces bone marrow mesenchymal stem cells towards cells with Schwann cell phenotype.Neurosci Lett. 2014;559:82-87.
[46] 张进,刘艳丽,孟秋菊,等.碱性成纤维细胞生长因子对人牙髓细胞生物学活性的影响[J].牙体牙髓牙周病学杂志, 2013,23(7): 450-453.
[47] 张戎,张勉,李成华,等.碱性成纤维细胞生长因子和血管内皮生长因子对人牙周膜干细胞体外增殖、迁移和黏附的影响[J].中华口腔医学杂志,2013,48(5):278-284.
[48] 李立恒,高燕飞.碱性成纤维细胞生长因子基因转染对人牙周韧带细胞的作用[J].中国组织工程研究,2012,16(33):6147-6151.
[49] 韦永珍,王秀颖,潘乙怀,等.碱性成纤维细胞生长因子对体外培养人牙髓细胞增殖和迁移的影响[J].现代口腔医学杂志, 2011, 25(2):126-130.
[50] 陈欣戬,闫福华,钟泉,等.碱性成纤维细胞生长因子基因转染对犬牙龈成纤维细胞的影响[J].中国组织工程研究与临床康复, 2009, 13(28):5444-5448.
[51] 余杰,曹金芳,金向青,等.碱性成纤维细胞生长因子对人牙周膜成纤维细胞在壳聚糖/磷酸三钙支架材料上生长的影响[J].实用医学杂志,2008,24(11):1868-1870.