Extrapericardial adipose-derived stem cells differentiate into myocardial cells

doi:10.3969/j.issn.2095-4344.2014.06.008

Abstract

Abstract:

BACKGROUND: Adult stem cells are capable of proliferation and differentiation, which are an important part of tissue engineering. Although several studies have demonstrated that human adipose-derived stem cells obtained from elective liposuction procedures represent the characters of mesenchymal stem cells and can be induced to differentiate into myocardial cells, less is known about the characters of extrapericardial adipose-derived stem cells and their differentiation into myocardial cells.

OBJECTIVE:To explore the culture of extrapericardial adipose-derived stem cells in vitro and their ability to differentiate into myocardial cells.

METHODS:The extrapericardial adipose tissues were obtained and then isolated, cultured and passaged in vitro. The expressions of cell surface specific antigens (CD44 and CD90) were detected by immunofluorescence assay at different time periods, and the fluorescence intensity was compared. After induced and cultured with myocardium induction medium for an optimal period, α-actin and cardiac troponin T expressions were detected by immunocytochemical detection and the phenotypic identifications were performed.

RESULTS AND CONCLUSION: During the in vitro culture, extrapericardial adipose-derived stem cells adhered to culture flask wall and proliferated strongly. The cells appeared to be shuttle-shaped and exhibit a vortex alignment. They were positive for CD44 and CD90, and the fluorescence intensity reached the peak at passages 3 and 4, but negative for CD34 and CD54. After induced and cultured with myocardium induction medium, the differentiated cells were muscle-like cells, the expression of α-actin and cardiac troponin T were positive, and the number of cells reached the peak at passages 3 and 4. These findings suggested that the extrapericardial adipose-derived stem cells from human represent the morphological and cellular immunological characters of mesenchymal stem cells, and they can be induced to differentiate into myocardial cells. In addition, there is the best induction period.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, pericardium, adipose tissue, myocytes, cardiac, cell differentiation

CLC Number:

R394.2

Zhang Jun, Zhang Yong, Lv Ji-yuan. Extrapericardial adipose-derived stem cells differentiate into myocardial cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 866-871.

Figures/Tables 2

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