Dexamethasone induces osteogenic differentiation and apoptosis of periodontal ligament stem cells

doi:10.3969/j.issn.2095-4344.2013.40.011

Abstract

Abstract:

BACKGROUND: Periodontal ligment stem cells are adult stem cells derived from dental tissues, which possess the capability of osteogenic differentiation. They are promising “seed cells” in bone tissue engineering.
OBJECTIVE: To investigate the effects of osteogenic induction medium on the osteogenic differentiation and early apoptosis of periodontal ligment stem cells.
METHODS: Periodontal ligment stem cells were isolated from primary periodontal ligament tissues and seeded on the plate at a density of 1×10⁴/cm². Osteogenic induction medium containing 1, 10 and 100 nmol/L dexamethasone, β-glycerophosphate and ascorbic acid were used. Alkaline phosphatase activity, mineral deposition staining and real time PCR were performed to assess the osteogenic capacity. AnnexinV/PI staining was used to detected apoptosis.
RESULTS AND CONCLUSION: Dexamethasone effectively promoted osteogenic differentiation of periodontal ligment stem cells, by up-regulating alkaline phosphatase activity, enhancing mineral deposition and increasing osteogenic related gene (osteonectin and type Ⅰ collagen) expression. Alkaline phosphatase activity and mineralized nodule detection showed that osteogenic effects of dexamethasone were gradient-dependent, and 100 nmol/L was the best concentration. Apoptosis detection results indicated that osteogenic differentiation of dexamethasone for periodontal ligment stem cells could also induce cell apoptosis to some extent.

Key words: stem cells, periodontal ligament, dexamethasone, alkaline phosphatase, apoptosis

CLC Number:

R394.2

Gu Zi-ya, Zhai Qiang, Lü Qiu-feng, Peng Can-xing, Liu Fang-fei, Peng Hui. Dexamethasone induces osteogenic differentiation and apoptosis of periodontal ligament stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(40): 7090-7095.

Figures/Tables 6

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