Biocompatibility of a novel chitosan-collagen scaffold and periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2013.38.010

Abstract

Abstract:

BACKGROUND: At present, collagen as a material in periodontal tissue engineering has some disadvantages such as poor mechanical strength and rapid degradation speed. Collagen combined with chitosan can improve above-mentioned problems.
OBJECTIVE: To evaluate the biocompatibility of a novel chitosan-collagen scaffold in vitro.
METHODS: Cytotoxicity of the extract of chitosan-collagen scaffold in different concentrations (100%, 75%, 50%, 25%) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human periodontal ligament cells at 4-6 passages were cocultured with the chitosan-collagen scaffold. Cell growth on the scaffold was observed. Changes in alkaline phosphatase activity were detected in periodontal ligament cells before and after coculture.
RESULTS AND CONCLUSION: The novel chitosan-collagen scaffold was made up of double layers with one dense layer and another loose layer. The grade of the cytotoxicity of the scaffold was from 0 to 1. Scanning electron microscope and histological observation demonstrated that cells grew well on the chitosan-collagen scaffold; the dense layer could prevent cells to migrate into the scaffold. There were no significant differences in alkaline phosphatase activity in human periodontal ligament cells before and 24 hours after combined culture (P > 0.05). Alkaline phosphatase activity in human periodontal ligament cells was greatly higher at 48 and 72 hours after combined culture compared with that before culture (P < 0.05). Above results indicated that the novel chitosan-collagen scaffold has a good biocompatibility and barrier function, and potential as a scaffold for periodontal tissue engineering.

Key words: biocompatible materials, mouth, chitosan, collagen, periodontal ligament

CLC Number:

R318

Tang Qian, Sun Wen-juan, Huang Nan-nan, Chen Xi, Ren Li, Hou Si-run. Biocompatibility of a novel chitosan-collagen scaffold and periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.38.010.

Figures/Tables 7

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