Chinese Journal of Tissue Engineering Research
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Hu Jing-qiong1, Ouyang Wei-xiang2, Li Hui-yu1, Wang Jun-feng1, Lu Cong1, Zhang Lan-nan1, Xu Hai-bo3, Chen Li-li4, Huang Shi-ang1
Received:
2012-10-02
Revised:
2012-12-20
Online:
2013-07-02
Published:
2013-07-02
Contact:
Chen Li-li, M.D., Professor, Dental Center, Wuhan Union Hospital, Wuhan 430022, Hubei Province, China
Corresponding author: Huang Shi-ang, M.D., Professor, Stem Cell Center, Wuhan Union Hospital, Wuhan 430022, Hubei Province, China
About author:
Hu Jing-qiong☆, M.D., Associate professor, Stem Cell Center, Wuhan Union Hospital, Wuhan 430022, Hubei Province, China
jingqionghu2006@gmail.com
Supported by:
The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of China*; Projects of International Cooperation and Exchanges, National Natural Science Foundation of China, No. 31110103905/C10030*; General Project of the National Natural Science Foundation of China, No. 81171386/H1808
CLC Number:
Hu Jing-qiong, Ouyang Wei-xiang, Li Hui-yu, Wang Jun-feng, Lu Cong, Zhang Lan-nan, Xu Hai-bo, Chen Li-li, Huang Shi-ang. Mesenchymal stem cells for the treatment of spinocerebellar ataxia[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.012.
2.1 参与者数量分析 纳入经临床、头颅MRI检查确诊的27例脊髓小脑性共济失调患者,其中6例行自体骨髓间充质干细胞治疗,细胞采集以及回输均为一次性,为1个疗程;21例行异体脐带间充质干细胞输注治疗,4 次为1个疗程。患者随访时间为1年,无随访脱落,按照意向性处理分析所有病例均进入结果分析。 2.2 脊髓小脑性共济失调的基因诊断 所有患者外周血DNA均采用PCR技术扩增ATXN1,3,7基因相应CAG重复序列并以1%琼脂糖凝胶电泳。正常阴性对照产生一正常条带(约200 bp),患者PCR扩增后产物电泳可见一正常条带(约200 bp)以及一异常条带 (有时有两条),明显大于300 bp,与脊髓小脑性共济失调阳性对照条带基本吻合,提示ATXN基因相应CAG重复序列异常增加,见图1A。对阳性病例扩增出的异常条带进行克隆测序。27例病例中11例脊髓小脑性共济失调3基因出现异常扩增,ATXN3基因相应CAG重复次数为50-78次,较正常值(13-40次)相比增加,见图1B。"
2.5 临床疗效 采用ICARS对患者治疗前后神经功能进行评定,两组患者治疗前ICARS评分大致相当,骨髓间充质干细胞治疗组治疗前ICARS(38.05±10.85)分;脐带间充质干细胞治疗组治疗前ICARS(39.56±14.52)分。6例脊髓小脑共济失调患者采用自体骨髓间充质干细胞治疗后效果均不明显,另外21例脊髓小脑共济失调患者行异体脐带间充质干细胞腰穿结合静脉输注治疗,治疗过程中以及治疗前后均未及明显不良反应,21例患者治疗前ICARS(39.56±14.52)分,治疗后1个月评分(36.28±14.76)分,差异无显著性意义。但3个月后患者自觉症状均有一定程度改善,ICARS评分(33.39±13.25)分,与治疗前相比明显降低(P < 0.05),见表2。"
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