Chinese Journal of Tissue Engineering Research
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Yang Zhong-lu, Wang Hui-shan
Received:
2013-02-07
Revised:
2013-06-05
Online:
2013-07-02
Published:
2013-07-02
About author:
Yang Zhong-lu★, Master, Attending physician, Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China
13309883275@163.com
CLC Number:
Yang Zhong-lu, Wang Hui-shan. Bone marrow mesenchymal stem cells from diabetic versus normal rats for treatment of myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.27.013.
贴壁细胞融合达75%以上,正常大鼠及糖尿病大鼠来源骨髓间充质干细胞均呈条索状梭形生长,糖尿病大鼠来源骨髓间充质干细胞所形成的集落明显小于正常大鼠来源骨髓间充质干细胞,且细胞伸展无力,较为扁平,细胞增殖能力较低。 2.3 大鼠骨髓间充质干细胞表面标抗原的检测结果 根据流式细胞检测结果,实验所培养的大鼠骨髓间充质干细胞表面标记物阳性表达情况表明,第2代贴壁细胞高水平表达骨髓间充质干细胞的标志物:96.12%的细胞表达CD90,98.14%的细胞表达CD44,97.51%的细胞表达CD29;而内皮细胞标志物CD31表达水平低,为3.25%;造血细胞标志物CD34表达率为1.17%;白细胞标志物CD45表达率为10.17%。证明实验所培养的大鼠骨髓来源的贴壁细胞是间充质干细胞。 2.4 正常与糖尿病大鼠来源骨髓间充质干细胞的生长曲线 结果显示正常大鼠来源骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞生长明显加快。因此,正常大鼠骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞在移植治疗心肌梗死过程中可能会由于细胞数量的优势而具有更好的疗效。见图3,表1。"
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