Root canal filling material added with tea polyphenols inhibits the generation of Candida albicans biofilm

doi:10.3969/j.issn.2095-4344.2013.21.025

Abstract

Abstract:

BACKGROUND: Candida albicans biofilms can influence the prognosis of root canal filling, and tea polyphenols can in vitro inhibit the Candida albicans biofilm, while the antibacterial mechanism may play the role through influencing or interfering the expression of resistance gene.
OBJECTIVE: To investigate the inhibitory effect of root canal filling material added with tea polyphenols on Candida albicans biofilm.
METHODS: Constant broth dilution method was used to determine the minimal inhibitory concentration of tea polyphenols in vitro inhibited Candida albicans biofilms, in order to identify the inhibitory effect. Then the expressions of Candida albicans resistance genes CDR1, CDR2 and MDR1 were detected with reverse transcriptase polymerase chain reaction after inhibited with tea polyphenols.
RESULTS AND CONCLUSION: The minimum inhibitory concentration of tea polyphenols used to inhibit Candida albicans biofilm was 11.5 mg/mL. Reverse transcriptase polymerase chain reaction results showed that expressions of related resistance genes CDR1 and CDR2 were decreased with the gradient increasing of drug concentration, and when the concentration reached to 25 mg/mL or above, the mRNA expression level was completely inhibited, while the concentration of the resistance drug has less effect on the expression of resistance drug MDR1. Tea polyphenols has inhibitory effect on Candida albicans biofilm, and has significant inhibitory effect on the expressions of experimental selected resistance genes CDR1 and CDR2.

Key words: biomaterials, biomaterial clinical practice, Candida albicans, biofilm, drug resistance, bacteria, tea polyphenols, tea pigment, root canal filling material, performance, endodontic sealers, antibacterial drugs, antibacterial effect, oral diseases, polymerase chain reaction, genotype

CLC Number:

R318

Xu Ying, Lü Qing, Kang Liang, Zhang Hui-ming. Root canal filling material added with tea polyphenols inhibits the generation of Candida albicans biofilm[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.025.

Figures/Tables 3

References

[1] Mah TF,O'Toole GA.Mechanisms of biofilm resistance to antimicrobial agents.Trends Microbiol.2001;9(1):34-39.

[2] Chandra J,Kuhn DM,Mukherjee PK,et al.Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance.J Bacteriol.2001;183(18): 5385-5394.

[3] Sun Z,Liu H. Yi Xue Yan Jiu Za Zhi. 2012;41(6):182-184.孙智,刘泓.新型根管充填材料的回顾研究[J].医学研究杂志, 2012, 41(6):182-184.

[4] Liu M,Li Z,Gao WX. Zhong Guo Lao Nian Xue Za Zhi. 2009; 29(9):1144-1145.刘明,李泽,高文信.两种根管充填材料的封闭性能比较[J].中国老年学杂志,2009,29(9):1144-1145.

[5] Wang P,Liang HY,Wu J,et al. Zhong Guo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 2010;14(12):2179-2182.王萍,梁焕友,吴坚,等.两种根管充填材料的封闭性能比较[J].中国组织工程研究与临床康复,2010,14(12):2179-2182.

[6] Qin H,Gong YQ,Xu ZH. Zhongguo Zughi Gongcheng Yanjiu Yu Linchuang Kangfu.2011;15(2): 232-235.秦晗,龚永庆,徐宏志.根管充填体外模型构建中X射线牙片的作用[J].中国组织工程研究与临床康复,2011,15(2): 232-235.

[7] Gu LS,Ling JQ. Guoji Kouqiang Yixue Zazhi.2006;33(5): 349-351.古丽莎,凌均棨.根管生物膜及其临床控制的研究进展[J].国际口腔医学杂志,2006,33(5):349-351.

[8] Qi FF,Wang S,Guo J.Xiandai Kouqiang Yixue Zazhi.2011; 25 (5): 359-362.齐芳芳,王山,郭洁.茶多酚、厚朴酚抑制早期人工根面龋作用的实验研究[J].现代口腔医学杂志,2011,25(5):359-362.

[9] Luo YN,Yu XY,Liu LQ.Hua Xue Shi Jie.2011;52(9):526-533.罗亚楠,于晓洋,刘立群.茶叶中茶多酚的提取及分析检测[J].化学世界,2011,52(9):526-533.

[10] Liao XL,Lang HY,Xu WF,et al.Fenxi Shiyanshi.2003;22(5): 70-72.廖晓玲,郎惠云,徐文峰,等.钼酸铵分光光度法测定绿茶中的茶多酚[J].分析试验室,2003,22(5):70-72.

[11] Tong Z,Dong L,Zhou L,et al.Nisin inhibits dental caries-associated microorganism in vitro.Peptides.2010;31 (11): 2003-2008.

[12] 中国知网.中国学术期刊总库[DB/OL].2012-08-10. https://www.cnki.net

[13] SCI数据库.Web of Sciencevia ISI Web of Knowledge[DB/OL]. 2012-08-10.http://ip-science.thomsonreuters.com/mjl

[14] Soustre J,Rodier MH,Imbert-Bouyer S,et al.Caspofungin modulates in vitro adherence of Candida albicans to plastic coated with extracellular matrix proteins.J Antimicrob Chemother. 2004;53(3):522-525.

[15] Bachmann SP,VandeWalle K,Ramage G,et al.In vitro activity of caspofungin against Candida albicans biofilms.Antimicrob Agents Chemother.2002;46(11):3591-3596.

[16] Alem MA,Douglas LJ.Effects of aspirin and other nonsteroidal anti-inflammatory drugs on biofilms and planktonic cells of Candida albicans.Antimicrob Agents Chemother.2004; 48(1): 41-47.

[17] Chandra J,Kuhn DM,Mukherjee PK,et al.Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance.J Bacteriol.2001;183(18): 5385-5394.

[18] Hazan Z,Zumeris J,Jacob H,et al.Effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves.Antimicrob Agents Chemother.2006; 50(12):4144-4152.

[19] Kuhn DM,Chandra J,Mukherjee PK,et al.Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces.Infect Immun.2002; 70(2):878-888.

[20] Chandra J,Patel JD,Li J,et al.Modification of surface properties of biomaterials influences the ability of Candida albicans to form biofilms.Appl Environ Microbiol.2005; 71(12):8795-8801.

[21] Andes D,Nett J,Oschel P,et al.Development and characterization of an in vivo central venous catheter Candida albicans biofilm model.Infect Immun.2004;72(10): 6023-86031.

[22] Tsang CS,Ng H,McMillan AS.Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness.Clin Oral Investig.2007;11(4):361-368.

[23] LaFleur MD,Kumamoto CA,Lewis K.Candida albicans biofilms produce antifungal-tolerant persister cells.Antimicrob Agents Chemother.2006;50(11):3839-3846.

[24] Szomolay B,Klapper I,Dockery J,et al.Adaptive responses to antimicrobial agents in biofilms.Environ Microbiol.2005;7(8): 1186-1191.

[25] Chang GJ,Wang AL.Shan Dong Yi Yao.2007;47(5):81-82.常桂娇,王艾琳.细菌生物膜及其与细菌耐药性关系的研究进展[J].山东医药,2007,47(5):81-82.

[26] Alem MA,Oteef MD,Flowers TH,et al.Production of tyrosol by Candida albicans biofilms and its role in quorum sensing and biofilm development.Eukaryot Cell.2006;5(10):1770-1779.

[27] Zhang L,Huang YC,Xi TF.Sheng Wu Yi Xue Gong Cheng Yu Lin Chuang.2006;10(3):194-196.张良,黄云超,奚廷斐.表皮葡萄球菌生物膜形成与生物材料感染[J].生物医学工程与临床,2006,10(3):194-196.

[28] Thurnheer T,Gmür R,Shapiro S,et al.Mass transport of macromolecules within an in vitro model of supragingival plaque.Appl Environ Microbiol.2003;69(3):1702-1709.

[29] Melchior MB,Fink-Gremmels J,Gaastra W.Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonic culture.J Vet Med B Infect Dis Vet Public Health.2006;53(7):326-332.

[30] Baillie GS,Douglas LJ.Iron-limited biofilms of Candida albicans and their susceptibility to amphotericin B.Antimicrob Agents Chemother.1998;42(8):2146-2149.

[31] Shi Q,Wang HN,Liu L.Wei Sheng Wu Xue Tong Bao.2008; 35(10):1633-1637.史巧,王红宁,刘立.细菌生物膜与耐药性相关性研究进展[J].微生物学通报.2008;35(10):1633-1637.

[32] Borriello G,Werner E,Roe F,et al.Oxygen limitation contributes to antibiotic tolerance of Pseudomonas aeruginosa in biofilms.Antimicrob Agents Chemother.2004;48(7): 2659-2664.

[33] Wang YX,Li HL,Li PY.Zhonghua Weishengwuxue He Mianyi Xue Zazhi.2003;23(6):428-431.王勇翔,李华林,李平洋.表皮葡萄球菌生物膜形成与ica操纵子的相关性研究[J].中华微生物学和免疫学杂志,2003,23(6): 428-431.

[34] Mukherjee PK,Chandra J,Kuhn DM,et al.Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols. Infect Immun.2003;71(8):4333-4340.

[35] Chen H,Fujita M,Feng Q,et al.Tyrosol is a quorum-sensing molecule in Candida albicans.Proc Natl Acad Sci U S A.2004; 101(14):5048-5052.

[36] Kohli A,Smriti,Mukhopadhyay K,et al.In vitro low-level resistance to azoles in Candida albicans is associated with changes in membrane lipid fluidity and asymmetry.Antimicrob Agents Chemother.2002;46(4):1046-1052.

[37] Khot PD,Suci PA,Miller RL,et al. small subpopulation of blastospores in candida albicans biofilms exhibit resistance to amphotericin B associated with differential regulation of ergosterol and beta-1,6-glucan pathway genes.Antimicrob Agents Chemother.2006;50(11):3708-3716.

[38] Nickerson KW,Atkin AL,Hornby JM. Quorum sensing in dimorphic fungi: farnesol and beyond.Appl Environ Microbiol. 2006;72(6):3805-3813.

[39] Xue P,Liu DC.Zhong Guo Yi Yao Dao Bao.2008;5(23):21-22.徐芃,刘东成.临茶多酚抗氧化和抑菌机制的研究[J].中国医药导报,2008,5(23):21-22.

[40] Cao YB,Xu Z,Zhang JD,et al.Zhongguo Xinyao Zazhi.2004; 13(2):102-105.曹永兵,徐铮,张军东,等.白色念珠菌耐药性产生的分子机制[J].中国新药杂志,2004,13(2):102-105.

[41] Morschhäuser J.The genetic basis of fluconazole resistance development in Candida albicans.Biochim Biophys Acta.2002;1587(2-3):240-248.