Biocompatibility of a novel injectable and degradable calcium phosphate cement

doi:10.3969/j.issn.2095-4344.2013.21.018

Abstract

Abstract:

BACKGROUND: A novel calcium phosphate bone cement has been proved to have good injectability, biomechanical properties, anti-collapsibility and in vitro degradability.
OBJECTIVE: To evaluate the biocompatibility of a novel injectable and degradable calcium phosphate cement.
METHODS: (1) In acute toxicity test, cement extracts were applied to mice by tail vein injection. (25) In pyrogen test, extracts were applied to rabbits by mainline. (3) In hemolysis test, extracts were added in the fresh anticoagulant blood of rabbits. (4) In delayed-type hypersensitivity test, two steps, induced and aroused were applied to guinea pigs. (5) In cytotoxicity test in vitro, extracts of cement, polyethylene, and phenol were added in L929 cells to evaluate cytotoxicity. (6) In mammal micronucleus test, extracts, normal saline and cyclophosphamide were applied to mice by intraperitoneal injection. (7) In muscular implantation test, muscle specimens were histologically evaluated.
RESULTS AND CONCLUSION: The cement-extracted liquid induced no acute toxicity in mice, no pyrogenic reaction in rabbits, no genetic toxicity in mice or allergic reaction in guinea pigs. This novel cement had a great biocompatibility and biological safety.

Key words: biomaterials, material biocompatibility, calcium phosphate cement, injectable, degradable, biocompatibility, osteoinductive materials, pyrogen reaction, hematocompatibility, biological safety, other grants-supported paper

CLC Number:

R318

Duan Xin, Liao Hong-xing, Zou Hua-zhang, Zhang Zi-ji, Zhang Hao, Zhang Zhi-qi, . Biocompatibility of a novel injectable and degradable calcium phosphate cement[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.018.

Figures/Tables 8

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