Biocompatibility of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release carriers

doi:10.3969/j.issn.2095-4344.2013.21.017

Abstract

Abstract:

BACKGROUND: The calcium sulfate is mostly used as carriers for antibiotics and osteogenic factors by now.
OBJECTIVE: To observe the in vitro delivery effect of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release system and the in vitro biocompatibility with bone marrow mesenchymal stem cells.
METHODS: Bone morphogenetic protein-2/injectable calcium sulfate cement slow-release scaffold was prepared and its release properties were detected. Bone marrow mesenchymal stem cells obtained from Sprague-Dawley rats were induced, cultured and proliferated in vitro, and then, the cells were seeded onto the bone morphogenetic protein-2/injectable calcium sulfate cement scaffolds (experimental group) or single injectable calcium sulfate cement scaffolds (control group).
RESULTS AND CONCLUSION: The release of bone morphogenetic protein-2 on the scaffolds of experimental group could last for 31 days. Rat bone marrow mesenchymal stem cells had a good biocompatibility with the bone morphogenetic protein-2/injectable calcium sulfate cement scaffolds. The adhesive rate, cell number and alkaline phosphatase activity at the same time point in the scaffolds of experimental group were significantly greater than those of the control group. Cells could be observed on each scaffold under a scanning electron microscope. In the experimental group, interconnected cell processes were fused, extracellular matrix was visible in the dense area, and the scaffold was surrounded by a large number of cells. The cells in experimental group grew better than the control group.

Key words: biomaterials, material biocompatibility, bone morphogenetic protein-2, injectable calcium sulfate cement, rat bone marrow mesenchymal stem cells, biocompatibility, other grants-supported paper

CLC Number:

R318

Chen Chen, Xu Xiao-feng . Biocompatibility of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release carriers[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.017.

Figures/Tables 5

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