Mannose-6-phosphate effects on collagen production and adhesion formation of the flexor tendon

doi:10.3969/j.issn.2095-4344.2013.08.017

Abstract

Abstract:

BACKGROUND: It has been confirmed that exogenous mannose-6-phosphate can inhibit type Ⅰ collagen production in tendon cells and prevent tendon adhesion after repair of flexor tendon injury.
OBJECTIVE: To investigate the preventive effect of mannose-6-phosphate on adhesion formation following flexor tendon repair.
METHODS: Seventy-two adult New Zealand White rabbit forepaws underwent sharp transection of the middle digit flexor digitorum profundus and immediate repair, and then were randomly divided into experimental (injected with mannose-6-phosphate) and control (injected with normal saline) groups. Tendons were harvested at 4 and 8 weeks postoperatively, and biomechanics testing, histological evaluation and scanning electron microscope observation were conducted. In situ hybridization method was performed to determine the mRNA expression of transforming growth factor beta 1 and type Ⅰ collagen.
RESULTS AND CONCLUSION: At 4 and 8 weeks after operation, the suture line was smooth, the gliding excursion ratio of the flexor tendon was higher, and the tendon sliding was constrained less in the experimental group as compared with the control group (P < 0.05). However, there was no significant difference in the tensile strength of tendon anastomosis between the two groups (P > 0.05). Scanning electron microscope and histological observation showed that collagen fibers arranged irregularly in the control group, but arranged regularly in the experimental group. The in situ hybridization examination revealed that transforming growth factor beta 1 and type Ⅰ collagen mRNA expression in the experimental group was lower than that in the control group (P < 0.05). These findings indicate that mannose-6-phosphate can inhibit the function of the transforming growth factor beta 1 effectively in the flexor tendon repair and prevent adhesion formation.

Key words: biomaterials, material biocompatibility, composite scaffold materials for tissue engineering, mannose-6-phosphate, transforming growth factor beta 1, type Ⅰ collagen, tendon adhesion, rabbits, provincial grants-supported paper, biomaterial photographs-containing pa

CLC Number:

R318

Zhu Hong, Cao Wen, Li Ya-juan, Yang Xuan-ying, Xia Chang-suo. Mannose-6-phosphate effects on collagen production and adhesion formation of the flexor tendon[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1430-1434.

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