Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (8): 1378-1383.doi: 10.3969/j.issn.2095-4344.2013.08.009

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Nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex treats esophageal cancer in vitro

Lu Xin-li1,2, Wang Yi-wei1, Zhang Dong-sheng2   

  1. 1 Jiangsu Jiankang Vocational College, Nanjing 210029, Jiangsu Province, China
    2 School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province, China
  • Received:2012-05-26 Revised:2012-07-12 Online:2013-02-19 Published:2013-02-19
  • Contact: Zhang Dong-sheng, Professor, Doctoral supervisor, School of Medicine, Southeast University, Nanjing 210009, Jiangsu Province, China zdszds1222@163.com
  • About author:Lu Xin-li★, Master, Lecturer, the 2011 Domestic Visiting Scholar of Southeast University, Jiangsu Jiankang Vocational College, Nanjing 210029, Jiangsu Province, China xllu_sky@163.com

Abstract:

BACKGROUND: Compared with traditional hyperthermia treatment, As2O3/Mn0.5Zn0.5Fe2O4 complex may have both the cytotoxicity effects and the targeted heating effects resulting from the radiofrequency-induced hyperthermia.
OBJECTIVE: To prepare nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex and to observe its growth inhibition effects on esophageal cancer cells Eca109.
METHODS: The As2O3/Mn0.5Zn0.5Fe2O4 complex was made using impregnation process. Its properties were described through transmission electron microscope, energy spectrometer, and atomic fluorescence spectrometer. In in vitro test, two plates of Eca109 cell lines were co-cultured with nanosized As2O3/Mn0.5Zn0.5Fe2O4, Mn0.5Zn0.5Fe2O4 or aqueous of As2O3, and then one of the plates was placed in an alternating magnetic field to rise to a therapeutic temperature, which is called magnetic fluid hyperthermia. The other plate of cells was cultured as usual.
RESULTS AND CONCLUSION: The nanosized As2O3/Mn0.5Zn0.5Fe2O4 were approximately global examined by transmission electron microscope. The As content measured by atomic fluorescence spectrophotometer was 0.012% to 0.066%. The proliferation ratio of the cells exposed to As2O3/Mn0.5Zn0.5Fe2O4 complex at the concentration of 5 μmol/L was significantly lower than that exposed to Mn0.5Zn0.5Fe2O4 alone or the negative control group (P < 0.05). The apoptosis rate of cells exposed to As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia was much higher than that exposed alone to Mn0.5Zn0.5Fe2O4 with magnetic fluid hyperthermia or As2O3 (P < 0.05). These findings indicate that As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia can significantly inhibit the proliferation of esophageal cancer cells.

Key words: biomaterials, nanobiomaterials, Mn0.5Zn0.5Fe2O4, As2O3/Mn0.5Zn0.5Fe2O4 complex, magnetic fluid hyperthermia, combined therapy, esophageal cancer, proliferation, apoptosis, the National Natural Science Foundation of China, biomaterial photographs-containing paper

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