In vitro osteogenic induction of bone marrow mesenchymal stem cells on bone morphogenetic protein-2/injectable calcium sulfate carrier

doi:10.3969/j.issn.2095-4344.2013.08.001

Abstract

Abstract:

BACKGROUND: Composite material of growth factor and scaffolds has the function of bone induction and conduction.
OBJECTIVE: To observe the effect of bone morphogenetic protein-2/injectable calcium sulfate carrier on the in vitro osteogenic induction of bone marrow mesenchymal stem cells.
METHODS: The second generation of bone marrow mesenchymal stem cells suspension in good growth state was collected from Sprague Dawley rats, and then divided into three groups. In the experimental group, the cells suspension was dropped on the surface of bone morphogenetic protein-2/injectable calcium sulfate composite; in the control group, bone morphogenetic protein-2 was injected into the cells suspension; cells in the blank control group were cultured normally.
RESULTS AND CONCLUSION: The number of the cells in three groups was increased with the prolonging of the incubation time, and the number of the cells in the experimental group was significantly larger than that in the control group and the blank control group (P < 0.05). The alkaline phosphatase activity at different culturing time points in the experimental group was higher than that in the control group and the blank control group (P < 0.05). In vitro experiment results showed that bone morphogenetic protein-2/injectable calcium sulfate carrier can promote the in vitro osteogenic induction of bone marrow mesenchymal stem cells.

Key words: biomaterials, tissue engineering bone materials, bone morphogenetic protein 2, injectable calcium sulfate, rat bone marrow mesenchymal stem cells, alkaline phosphatase, other grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Xu Xiao-feng, Chen Chen, Chen Hai-ning, Chen Liang, Zhang Zhi-jian. In vitro osteogenic induction of bone marrow mesenchymal stem cells on bone morphogenetic protein-2/injectable calcium sulfate carrier[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1331-1520.

Figures/Tables 5

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