Construction of a co-culture system for human bone marrow stromal cells-osteoblasts in vitro

doi:10.3969/j.issn.2095-4344.2013.06.006

Abstract

Abstract:

BACKGROUND: Bone marrow stromal cells and osteoblasts play a major role during bone repair and reconstruction and the two are closely associated with each other functionally.
OBJECTIVE: To establish a co-culture system for bone marrow stromal cells-osteoblasts in vitro so as to investigate the features of both kinds of cells in one system.
METHODS: Human bone marrow stromal cells and osteoblasts were primary isolated and these two kinds of cells were co-cultured in a Transwell system to establish a co-culture system for human bone marrow stromal cells-osteoblasts. The proliferation, apoptosis and other features of human bone marrow stromal cells and osteoblasts were determined by MTT assay, acridine orange staining and alkaline phosphatase activities.
RESULTS AND CONCLUSION: The co-culture system for human bone marrow stromal cells and osteoblasts promoted the proliferation of osteoblasts, increased alkaline phosphatase expression, inhibited apoptosis of bone marrow stromal cells and promoted the aggregation of bone marrow stromal cells. These results indicate that in the co-culture system for human bone marrow stromal cells and osteoblasts, bone marrow stromal cells can increase the osteogenic ability of osteoblasts by accelerating their proliferation; in addition, osteoblasts can lower the apoptosis of bone marrow stromal cells and strengthen their osteogenic differentiation. These findings suggest a close functional association between human bone marrow stromal cells and osteoblasts.

Key words: stem cells, bone marrow-derived stem cells, bone marrow stromal cells, osteoblasts, co-culture, bone repair, bone regeneration, proliferation, apoptosis, bone tissue engineering, National Natural Science Foundation of China, stem cell photographs-containing paper

CLC Number:

R394.2

Yang Chun-lu, Zhao Yong, Chen Jian-ting. Construction of a co-culture system for human bone marrow stromal cells-osteoblasts in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 980-984.

Figures/Tables 3

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