Heat-shocked sweat gland cells induce the phenotype transformation of human bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.06.007

Abstract

Abstract:

BACKGROUND: Co-culture of bone marrow mesenchymal stem cells with heat-shocked sweat gland cells or non-shocked sweat gland cells does not influence the phenotype transformation of human bone marrow mesenchymal stem cells.
OBJECTIVE: To establish heat-shocked sweat gland cell models in vitro and a co-culture system of bone marrow mesenchymal stem cells and sweat gland cells. Morphological and phenotypic changes in bone marrow mesenchymal stem cells before and after co-culture were analyzed. The feasibility of bone marrow mesenchymal stem cells differentiating into sweat gland cells was investigated.
METHODS: Bone marrow mesenchymal stem cells and sweat gland cells were isolated, cultured, amplified and identified in vitro. In vitro heat-shocked sweat gland cell models were established and further incubated for 3-5 days. The supernatants were collected as conditioned medium to induce the differentiation of bone marrow mesenchymal stem cells. Morphology of bone marrow mesenchymal stem cells was compared between 5 and 10 days of co-culture. Phenotypic changes of bone marrow mesenchymal stem cells after 10 days of co-culture were detected by immunohistochemical staining and flow cytometry.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were positive for surface markers CD29, CD44 and CD105, and sweat gland cells were positive for surface markers CK7, CK8, CK18, CK19 and CEA. After induction, the differentiated cells were positive for CEA, CK7, CK8 and CK19. However, positive expression of CEA, CK7, CK8 and CK19 was not detected in the differentiated cells after co-cultured with non-heat-shocked sweat gland cells. Through the flow cytometry, the positive expression rate of CEA, CK7, CK8 and CK19 was 60.67%, 53.34%, 54.11% and 58.62%, respectively in the differentiated cells. These findings suggest that bone marrow mesenchymal stem cells were successfully induced and phenotype of sweat gland cells was acquired. By the cross-mesoderm way, bone marrow mesenchymal stem cells from the mesoderm can convert into sweat gland-like cells through establishing a proper in vitro culture system.

Key words: stem cells, bone marrow-derived stem cells, bone marrow mesenchymal stem cells, heat shock, sweat gland cells, mesoderm, co-culture, phenotype transformation, flow cytometry, immunohistochemistry, trauma repair, tissue regeneration, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Ai Li, Weng Li-xin, Sun Tong-zhu. Heat-shocked sweat gland cells induce the phenotype transformation of human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 985-991.

Figures/Tables 7

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