Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (10): 1546-1551.doi: 10.3969/j.issn.2095-4344.1626

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Effects of domestic porous tantalum on expression of collagen type I, tissue transglutaminase 2 and calcium-binding protein A4 in MG63 cells

Chen Jingjing1, Wang Qian1, Cui Yishuang1, Teng Xuefeng1, Zhang Hui2, Li Qijia1, 3   

  1. 1Medical Research Center, 3School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China; 2First Department of Joint, Second Hospital of Tangshan, Tangshan 063000, Hebei Province, China
  • Received:2018-11-12 Online:2019-04-08 Published:2019-04-08
  • Contact: Li Qijia, Professor, Chief physician, Master supervisor, Medical Research Center, North China University of Science and Technology, Tangshan 063210, Hebei Province, China; First Department of Joint, Second Hospital of Tangshan, Tangshan 063000, Hebei Province, China
  • About author:Chen Jingjing, Master candidate, Medical Research Center, North China University of Science and Technology, Tangshan 063210, Hebei Province, China
  • Supported by:

    the Project supported by the Ministry of Science and Technology of China, No. 2012BAE06B03; Hebei Province Science and Technology Support Project, No. 16277776D (to LQJ); Hebei Provincial Medical Research Project, No. 20160225; the Project Supported by the Foundation of Doctoral Research of North China University of Science and Technology, No. 28606299 (to WQ); the Project Supported by Hebei Provincial Health and Family Planning Commission, No. 20180733 (to WQ)

Abstract:

BACKGROUND: At present, there is evidence that domestic porous tantalum has good biocompatibility and osteogenic properties, but the specific osteogenic mechanism and its effect on osteogenic factors are still unclear.

OBJECTIVE: To observe the effects of domestic porous tantalum materials on the expression of collagen type I, tissue transglutaminase-2 and calcium-binding protein A4 in MG63 cells.
METHODS: MG63 cells in logarithmic growth phase were inoculated onto 24-well plates and cultured in three groups: in blank group, conventional medium was added; in tantalum extract group, porous tantalum material extract was added; and in tantalum scaffold group, porous tantalum material and conventional medium were added. On 1, 3, 5, 7 and 9 days of culture, the cell proliferation of each group was detected by cell counting kit-8 method. On 5 days of culture, the levels of collagen type I, tissue transglutaminase-2 and calcium-binding protein A4 secreted by MG63 cells in each group were detected by ELISA. Western blot assay was used to detect the expression of three proteins in each group.

RESULTS AND CONCLUSION: (1) With the prolongation of culture time, the number of cells in each group increased gradually. There was no difference in cell proliferation among the three groups at different time points (P > 0.05). (2) The secretory levels of collagen type I and tissue transglutaminase-2 in the tantalum scaffold group were significantly higher than those in the blank group and tantalum extract group  (P < 0.05), while the secretion of collagen type I and tissue transglutaminase-2 in the tantalum extract group was significantly higher than that in the blank group (P < 0.05). The secretion of calcium-binding protein A4 in the tantalum scaffold group was significantly lower than that in the other two groups (P < 0.05). (3) The expression of collagen type I and tissue transglutaminase-2 protein in the tantalum scaffold group was significantly higher than that in the blank group and tantalum extract group (P < 0.05), while the expression of collagen type I and tissue transglutaminase-2 protein in the tantalum extract group was significantly higher than that in the blank group (P < 0.05). The expression of calcium-binding protein A4 in the tantalum scaffold group was significantly lower than that in the blank group and tantalum extract group (P < 0.05). To conclude, domestic porous tantalum materials could promote the secretion of collagen type I and tissue transglutaminase-2 by MG63 cells, and inhibit the secretion of calcium-binding protein A4.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Tantalum;, Collagen Type I, Calcium-Binding Proteins;, Osteoblasts, Tissue Engineering

CLC Number: