Microleakage from the crown to the root canal after post space preparation with different timing and remaining length

doi:10.3969/j.issn.2095-4344.1629

Abstract

Abstract:

BACKGROUND: Poor root canal filling or poor post-core crown restoration can cause microleakage between the implant material and the tooth, leading to secondary infection of the periapical tissue and affecting long-term effect of tooth restoration.

OBJECTIVE: To analyze the microleakage in a glucose penetration model when post space preparation is performed with different timing and remaining lengths.

METHODS: Eighty-six freshly extracted mandibular premolars from the Orthodontics Department of Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University were randomly divided into eight groups: positive control group (n=10) undertook root canal preparation; negative control group (n=10) undertook root canal preparation and filling but not post space preparation; A1, B1 and C1 groups (n=11 per group) were subjected to root canal filling immediately followed by post space preparation with the filling material of 4 mm, 5 mm, and 6 mm in length, respectively; A2, B2 and C2 groups were subjected to root canal filling and 1 week after filling, the three groups underwent post space preparation with the filling material of 4, 5 and 6 mm in length, respectively. At 48 hours after post space preparation, the integration of root canal wall and filling material was observed by scanning electron microscopy. The glucose microleakage model was used to detect the amount of glucose leaking from the crown to the root in each group.

RESULTS AND CONCLUSION: (1) Under the scanning electron microscope, the fillings were most tightly bonded to the root canal wall in C1, while microcracks were most apparent in A2. (2) According to the measurement of glucose penetration model, A2 showed more microleakage than A1 (P < 0.05), B2 showed more microleakage than B1 (P < 0.05), and there was no statistically significant difference between C1 and C2 (P > 0.05). No significant difference was found among A1, B1 and C1 (P > 0.05), B2 showed no statistical difference in the microleakage from A2 and C2 (P > 0.05), but A2 showed more microleakage than C2 (P′ < 0.017). These results indicate that immediate post space preparation is superior to delayed preparation in reducing the microleakage. For immediate post space preparation, the remaining length of the filling material has no effect on the microleakage, but for delayed preparation, the filling material of at least 5 mm in length should be preserved.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Root Canal Therapy, Root Canal Preparation, Post and Core Technique, Tissue Engineering

CLC Number:

R459.9

R329.3

Wang Jieqi, Zheng Meihua, Wu Hong, Li Xiaoyu, Xie Wenqiang. Microleakage from the crown to the root canal after post space preparation with different timing and remaining length[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(10): 1552-1557.

Figures/Tables 1

2.1 扫描电镜观察结果扫描电镜显示，C1组根管充填物与根管壁最紧密连接，根充物与根管壁间微缝隙最小，根管封闭剂聚合均匀、致密，充填物未见明显变形或破碎；A1组、B1组、C2组根管充填物与根管壁结合情况与C1组相似，牙胶尖未见明显变形，但充填物与根管壁间微缝隙较C1组大，且缝隙中可见少量颗粒状疏松分布的封闭剂；B2组充填物与根管壁间微缝隙较B1组明显，且根管封闭剂呈团块状分布于缝隙；A2组根管充填物与根管壁间存在最明显微缝隙，充填物移位，封闭剂变形、碎裂，封闭剂-牙胶尖及封闭剂-根管壁界面间均存在明显裂隙，不能紧密结合，见图2。 2.2 阳性对照组和阴性对照组葡萄糖冠向微渗漏情况阳性对照组于第1天即可观察到葡萄糖从牙体冠方向根方渗漏，且呈快速上升趋势；阴性对照组观察至28 d无葡萄糖渗漏。说明该装置密封性良好，葡萄糖溶液可经根管系统从冠方流向根方，该模型灵敏、可靠。 2.3 各实验组冠向微渗漏的葡萄糖浓度统计结果各实验组标本均有微渗漏发生，A1组葡萄糖微渗漏量为(4.77±3.61) mmol/L，A2组为(21.71±9.08) mmol/L，B1组为(3.55±3.33) mmol/L，B2组为(7.83±6.32) mmol/L，C1组为(2.86±1.83) mmol/L，C2组为(5.27±3.67) mmol/L。经统计学分析，A2组微渗漏量高于A1组(Z=3.781， P < 0.05)；B2组微渗漏量高于B1组(Z=2.118，P < 0.05)；C1组和C2组微渗漏量无差异(Z=1.441，P > 0.05)；A1组、B1组、C1组微渗漏量无差异(χ2=0.673，P > 0.05)；A2组、B2组、C2组微渗漏量有差异(χ2=15.518，P < 0.05)，组间两两比较，A2组与B2组微渗漏量无差异(Z=2.722， P′> 0.017)、B2组与C2组微渗量无差异(Z=1.097，P′> 0.017)，A2组微渗漏量大于C2组(Z=3.781，P′< 0.017)。 "

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