Expression of myeloid differentiation factor 88 in a rat model of experimental periapical periodontitis

doi:10.3969/j.issn.2095-4344.1049

Abstract

Abstract:

BACKGROUND: Studies have found that myeloid differentiation factor 88 is expressed in rat dental follicles and affects the eruption of teeth and the number of osteoclasts.
OBJECTIVE: To further verify the expression of myeloid differentiation factor 88 in an experimental rat model of periapical periodontitis.
METHODS: Twenty-five male Sprague-Dawley rats, 6 weeks of age, were provided by the SPF Animal Experimental Center of Dalian Medical University in China. The pulp of the first mandibular molar was exposed to establish the rat model of experimental periapical periodontitis in each rat. Endodontic pulp exposure 0 week was set as control group, while endodontic pulp exposure 1, 2, 3, 4 weeks as experimental group. Five rats were randomly selected at each time point after exposure of the endodontic pulp. Then, paraformaldehyde fixation of the mandible, decalcification, and pathological frozen sections were mode. Hematoxylin-eosin staining and immunohistochemical staining were used to observe the pathological changes of the periapical tissues. RT-PCR was used to detect the expression of myeloid differentiation factor 88 in the rat model of experimental periapical periodontitis.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining results showed inflammatory infiltration in the apical area of the experimental group, and spread around, indicating that we successfully established the rat model of experimental periapical periodontitis. (2) Immunohistochemical staining results showed the positive expression of myeloid differentiation factor 88 in the periapical inflammation area at 1, 2, 3, 4 weeks after modeling. The positive expression of myeloid differentiation factor 88 significantly increased within 1-3 weeks, peaked at 3 weeks, but decreased at 4 weeks. There were only a small number of myeloid differentiation factor 88 positive cells in the control group. (3) RT-PCR results showed that the mRNA expression of myeloid differentiation factor 88 increased gradually from 1 week to 3 weeks, and then decreased at 4 weeks. To conclude, myeloid differentiation factor 88 is expressed in the animal model of experimental periapical periodontitis and shows a tendency of expression over time. Myeloid differentiation factor 88 may be involved in the occurrence of periapical periodontitis and the resorption of alveolar bone in periapical periodontitis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Myeloid Differentiation Factor 88, Periapical Periodontitis, Models, Animal, Tissue Engineering

CLC Number:

R446，R318

Zuo Meina1, Wang Lina1, Han Shujuan2, Dong Ming3, Niu Weidong1. Expression of myeloid differentiation factor 88 in a rat model of experimental periapical periodontitis [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1068-1072.

Figures/Tables 4

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